16S rRNA基因测序在法医学中的研究进展

Progress in the 16S rRNA Gene Sequencing in Forensic Science

法医微生物是目前法医学研究的前沿热点之一。16S rRNA基因由于其保守性与特异性并存的特点,是法医学鉴定的理想标记。伴随着高通量测序技术的快速发展,对微生物的研究已逐步应用于环境、医疗等多个领域。在法医学领域,以16S rRNA基因测序为代表的法医微生物研究成果也逐步应用于法医学实践中的生物检材鉴定、个体识别、死亡时间推断、地域推断等方面,为案件的侦查提供线索,为传统方法作为补充和辅助。本文阐述了16S rRNA基因测序应用于法医学领域的研究方法和相关测序技术,综述了其在法医学领域的研究进展,探讨了16S rRNA在法医学中的应用价值和潜力。

Forensic microorganism is one of the hotspots of forensic science research.Due to its conservatism and specificity,the 16S rRNA gene is found to be an ideal marker for forensic identification.With the rapid development of high throughput sequencing technology,the research on microorganisms has been gradually applied to many fields such as environment and health care.In the field of forensic science,the results of forensic microbiology research,represented by 16S rRNA gene sequencing,are also gradually applied to forensic practice,such as biological samples identification,individual identification,postmortem interval estimation,and regional inference,which not only provide clues for the investigation of cases but also complement and assist traditional methods.This paper describes the research methods and related sequencing technologies of 16S rRNA gene sequencing,summarizes its research progress,and discusses the application value and potential of 16S rRNA in forensic science.