动脉粥样硬化靶向CD47抗体-壳聚糖-透明质酸纳米聚电解质复合物的合成及理化特性检测

Synthesis and Physicochemical Properties of CD47/Chitosan-hyaluronic Acid Polyelectrolyte Complexes Targeting Nanoparticles for Atherosclerosis

目的利用壳聚糖(chitosan,CS)、透明质酸(hyaluronic acid,HA)有效吸附CD47抗体,合成针对动脉粥样硬化的靶向纳米给药载体。方法在水和PBS溶液中,以壳聚糖作为聚阳离子,透明质酸作为聚阴离子,二者发生电荷中和,合成壳聚糖-透明质酸聚电解质复合物纳米颗粒(chitosan-hyaluronic acid polyelectrolyte complex nanoparticles,CSHA-PECS)。以扫描电镜、动态光散射及迁移率电泳验证合成的CS-HA-PECS的表观特征及理化特性。将CD47抗体吸附于CS-HA-PECS表面形成稳定的CD47/CS-HA-PECS靶向纳米颗粒。结果成功合成CS-HA-PECS,其在水和PBS溶液中均具有长期的稳定性;CS-HA-PECS的聚合过程及其物理化学性质受到电荷混合比和聚合物浓度等参数的影响;CD47抗体可稳定高效地吸附于CS-HA-PECS的表面,形成稳定的CD47/CS-HA-PECS纳米颗粒复合物,其平均粒径在375～620nm之间,Zeta电位为正。结论成功合成了CD47/CS-HA-PECS靶向纳米颗粒,为动脉粥样硬化靶向纳米给药奠定了基础。

Objective In this study,chitosan(CS)andhyaluronicacicK HA)wcrcuscd tocffcctivclyabsorb thcCI>l 7anti-body to synthesize the targeted nano-drug carrier for athcrosclcrosi..Methods With chitosan as thc polycation and hyaluronic acid as thc polyanion,they reacted with cach other in water and PBS solution,and their chargc was neutralized and chitosan-hy-aluronic acid polyclcctrolytc complcxcs(CS-H A PEC.)synthesized.Thc apparent characteristics andphysicochcmical properties of the synthesized CS-HAPEC nanoparticlcs were verified by scanning clcctronmicroscopy,dynamic light scattering andmiobili-ty electrophoresis.CI47 antibody was absorbed on the surface of CS-HA PECnanoparticlcs to forrn stable CI47/’CS-HA PEC targeting nanoparticlcs.Results The CS-H A PEC nano-polyclcctrolyte complex was successfully synthesized,whichhad longterm stability in water and PBS solution.T'he polymerization process andphysiochcmical properties of CS-HA PECnanoparti-clcs were affected by parameters such as charge mixing ratio and polymer concentration.CH 7 antibody could be stably and efti-cicntly absorbed on the surface of CS-HAPEC nanoparticlcs to forrn a stable CI)4 7/CS-HA PEC nanoparticlc cornposite with an average particle size of 375-620 nm and a positive zeta potentia..Conclusion CI)4 7/CS-H A PEC targeting nanoparticlcswcre successfully synthesized in this study,which lays a solid foundation for atherosclerosis-targeting nano-drug delivery.