正交试验法优化平卧菊三七多糖GPP-20双氧水脱色工艺研究

Optimization of Decolorization Condition of Gynura procumbens Polysaccharide GPP-20 Using H_2O_2 Method by Orthogonal Test

用双氧水对平卧菊三七多糖GPP-20(20%乙醇沉淀物)进行脱色处理,以脱色率和多糖保留率双重指标共同评价脱色效果。首先通过单因素试验考察双氧水加入量、脱色温度、脱色时间和pH值对脱色效果的影响,然后采用L9(34)正交试验进一步优化出GPP-20的最佳脱色条件。结果表明,在温度为70℃,双氧水体积分数为15%,pH值为9的条件下脱色4 h,GPP-20的脱色率和多糖保留率分别为(88.00±0.45)%和(71.32±0.67)%,综合评分79.66±0.32,达到较好的脱色效果。

Hydrogen peroxide was applied to study the decolorization conditions of GPP-20,a polysaccharide isolated from Gynura procumbens by 20%ethanol precipitation method,and the decolorization rate and polysaccharide retention rate were calculated to evaluate the comprehensive decolorization effect.The addition amount of hydrogen peroxide,decolorization temperature,decolorization time and pH value were investigated by single factor experiment,and the L9(34)orthogonal test was used to optimize the optimum decolorization condition.The results showed that when the decolorizatemperature was 70℃,addition amount of hydrogen peroxide was 15%,pH value was 9,and decolorization time was 4 h,the decolorization rate and polysaccharide retention rate were(88.00±0.45)%and(71.32±0.67)%,respectively.The comprehensive score was calculated to be(79.66±0.32),and under this condition the best decolorization effect was achieved.