复合酶提取金樱子根多糖工艺的优化及其抗氧化活性

Polysaccharides from Rosa laevigata roots,their compound enzyme extraction technique optimization and the antioxidant activity

目的优化复合酶提取金樱子根多糖工艺,并评价其抗氧化活性。方法在单因素试验基础上,以酶添加量、酶解pH、酶解时间、液料比为影响因素,多糖得率为评价指标,响应面法优化提取工艺。再测定多糖对DPPH、·OH、O-2·自由基的清除作用。结果最佳条件为酶添加量2. 1%,酶解pH 4. 4,酶解时间49 min,液料比16∶1,酶解温度45℃,多糖得率141. 59 mg/g。3. 0 mg/m L多糖对DPPH、·OH、O-2·自由基的清除率分别为87. 81%、86. 14%、86. 37%,IC50分别为0. 935、1. 274、1. 521 mg/m L。结论该方法简便可行,可用于复合酶提取抗氧化活性较强的金樱子根多糖。

AIM To optimize the compound enzyme extraction technique for polysaccharides from Rosa laevigata Michx.roots and to evaluate the antioxidant activity.METHODS With enzyme addition,enzymolysis pH,enzymolysis time and liquid-solid ratio as influencing factors,polysaccharides yield as an evaluation index,response surface method was applied to optimizing the extraction on the basis of single factor experiment.Subsequently the scavenging effects of polysaccharides on DPPH,·OH,O2·free radicals were determined.RESULTS The optimal conditions were determined to be 2.1%for enzyme addition,4.4 for enzymolysis pH,49 min for enzymolysis time,16:1 for liquid-solid ratio,and 45 d for enzymolysis temperature,the polysaccharides yield was 141.59 mg/g.The scavenging rates of polysaccharides(3.0 mgmL)on DPPH,·OH,O2·free radicals were 87.81%,86.14%,86.37%witli the IC50 values of 0.935,1.274,1.521 mgmL,respectively.CONCLUSION This simple and feasible method can be used for the compound enzyme extraction for polysaccharides with strong antioxidant activity from R.laevigata roots.