伐地那非预处理对精子冷冻效果的影响

Effect of vardenafil pretreatment on the activity of post-thaw sperm

目的·探索伐地那非预处理对精子冷冻复苏后活力的影响。方法·在上海市人类精子库随机收集30例捐献者的精液标本,并将收集的每例精液平均分成6份,1份用于冷冻前指标测定,其余5份分别用0、0.4、4.0、40.0、400.0μg/mL的伐地那非孵育5 min。按精液与保护剂2:1的比例添加保护剂,采用一步熏蒸法进行冷冻,冷冻复苏后分别检测每份标本的前向运动精子、精子活力及正常精子形态等参数。结果·复苏后,0、0.4、4.0、40.0、400.0μg/mL伐地那非处理后的前向运动精子百分率分别为(41.47±9.80)%、(42.57±9.60)%、(47.77±8.55)%、(37.27±8.47)%、(26.37±6.99)%。与对照组(0μg/mL伐地那非组)比较,4.0μg/mL伐地那非处理后的精子复苏后活力有显著改善,差异有统计学意义(P=0.034)。结论·适当浓度的伐地那非预处理精液能提高精子冷冻复苏后的活力;然而伐地那非浓度过大,可能对精子有毒害作用。

Objective·To explore the effect of vardenafil pretreatment on the post-thaw sperm activity.Methods·Semen samples were randomly collected from 30 donors in Shanghai Human Sperm Bank.Then,each semen sample was aliquoted into six parts.One was immediately assayed for sperm activity without any treatment as the original control.The other five parts were incubated with vardenafil at 0,0.4,4.0,40.0,and 400.0μg/mL respectively for five minutes.The protective agent was added according to the semen:protective agent ration(2:1),and the fumigation method was used for cryopreservation.After thawing,each specimen was examined for various parameters,including progressive motility,total motility and normal sperm morphology.Results·After thawing,the progressive sperm rates were(41.47±9.80)%,(42.57±9.60)%,(47.77±8.55)%,(37.27±8.47)%,and(26.37±6.99)%in the groups treated with 0,0.4,4.0,40.0,and 400.0μg/mL of vardenafil,respectively.Compared to the control group(0μg/mL of vardenafil),4.0μg/mL of vardenafil could significantly improve the post-thaw sperm motility(P=0.034).Conclusion·Vardenafil pretreatment can improve the activity of the postthaw sperm;however,it may be toxic to sperm at the high concentration.