基于piggyBac转座子系统的西方蜜蜂Rubia基因转移研究

PiggyBac Transposon System-Mediated Transfer of Rubia Gene Transfer in Apis mellifera

为探讨piggy Bac载体在西方蜜蜂(Apis mellifera)中的基因转移功能,并检测其可行性,研究以西方蜜蜂为实验材料,利用piggy Bac转座子系统将外源性Rubia红色荧光蛋白基因转移至西方蜜蜂受精卵中,分析检测外源基因的整合与荧光表达情况。PCR扩增和测序分析结果显示piggy Bac转座子能够将外源性Rubia红色荧光蛋白基因整合到蜜蜂基因组上,且在显微注射组中检测到微弱的红色荧光蛋白表达。本研究证实piggy Bac转座子系统可将外源性基因植入蜜蜂基因组。为今后的蜜蜂转基因基础研究和分子育种技术开发提供支撑。

To explore the gene transfer function of piggyBac transposon in western honeybees(Apis mellifera)and to further examine its feasibility,Apis mellifera was used as an experimental material.The piggyBac transposon system was used to transfer the exogenous red fluorescent protein gene Rubia into the honeybee zygotes for the analysis and detection of foreign gene integration and fluorescence expression in honeybees.PCR amplification and sequencing analysis showed that the piggyBac transposon was able to integrate the exogenous Rubia red fluorescent protein gene into the honeybee genome.Fluorescence microscopy detection also showed a weak red fluorescent protein expression.This study confirms that the piggyBac transposon system could implant exogenous gene into honeybee genome.This would provide a basis for the future development of honeybee transgenic research and exploitation in molecular breeding.