低温对茄子幼苗生理特性的影响及耐冷性指标的筛选

Effects of Low Temperature on Physiological Properties of Eggplant Seedlings and Selection of Cold-Tolerance Indicators

以茄子幼苗为材料,探讨低温胁迫对茄子幼苗生理生化特性的影响,并对各指标进行相关性分析。结果表明:低温胁迫使茄子幼苗叶片光合色素含量降低;增加了茄子幼苗丙二醛(MDA)、脯氨酸、可溶性蛋白和可溶性糖含量,提高了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,且电导率值显著增大。恢复常温,光合色素含量都有不同程度升高,但低于对照,其他指标均降低,仍高于对照。相关性结果分析表明:所测各指标之间呈极显著正相关,且电导率与丙二醛、可溶性糖之间相关系数最大。研究结果表明,10℃/5℃(昼/夜)低温环境严重影响茄子幼苗的正常代谢,低于10℃/5℃(昼/夜)茄子幼苗保护系统破坏严重,不能够恢复正常生长,电导率、丙二醛、可溶性糖含量可作为茄子幼苗耐低温能力可靠的鉴定指标。

Effects of low temperature stress on the physiological and biochemical properties as well as the antioxidant enzyme activities of the eggplant seedlings and the correlation among the indicators for the cold-tolerance of the plants were studied.The results indicated that cold stress reduced the content of chlorophyll in leaves but increased those of MDA,proline,soluble protein and soluble sugar in the seedlings.Meanwhile,the electric conductivity and the activities of SOD,POD and CAT also increased significantly.By moving the stressed seedlings back into normal temperature,the chlorophyll content increased slightly but remained lower than control,while the other indicators declined to the levels still higher than those of control.There were ery significant correlations existed among the indicators.The highest correlation coefficient was found between the electric conductivity and the MDA or soluble sugar content.It appeared that a day/night low temperature exposure at 10℃/5℃would irreversibly damage the normal metabolism of the seedlings.The electric conductivity,MDA and soluble sugar seemed to be appropriate for evaluating the cold-tolerance of eggplant seedlings.