产pseurotin A内生真菌GYP8的鉴定

Identification of Pseurotin A-producing Endophytic Fungus GYP8

[目的]研究甘草内生真菌GYP8活性代谢物,丰富pseurotin A植物内生真菌资源库。[方法]利用HPLC法对GYP8发酵液进行分析;采用柱色谱分离法对GYP8活性成分进行分离、纯化;采用形态特征观察法及18Sr DNA序列分析对内生真菌GYP8进行菌种鉴定。[结果]内生真菌GYP8发酵液中含有pseurotin A,经鉴定该菌为烟曲霉(Aspergillus fumigatus)。[结论]GYP8是pseurotin A产生菌,该菌的获得可为pseurotin A成分的生产提供新方法。

[Objective]To study the active metabolites of endophytic fungi GYP8 from Glycyrrhiza uralensis and enrich the pseurotin A endophyte resource pool.[Method]The fermentation broth of GYP8 was analyzed by HPLC.The positive components of GYP8 were separated and purified by column chromatography.Strain identification of the endogenous fungi GYP8 was performed by morphological observation and 18SrDNA sequence analysis.[Result]Compound pseurotin A was isolated from the fermentation broth of GYP8 and endophytic fungus GYP8 was identified as Aspergillus fumigatus.[Conclusion]GYP8 is a pseurotin A-producing endophytic fungus,which provides a new means for development and application of pseurotin A.