LAIR1基因3’端非翻译区多态性与胃癌发病风险的关系研究

Association of 3'UTR polymorphisms of LAIR1 gene with susceptibility of gastric cancer

目的分析人白细胞相关免疫球蛋白样受体1(LAIR1)基因3’端非翻译区(3’UTR)多态性与胃癌发病风险的关系。方法选取经胃镜下组织活检确诊的121例胃癌患者为胃癌组,另取同期收治且年龄、性别相匹配的126例慢性胃炎患者为对照组。采用一代测序法检测并比较两组患者LAIR1基因3’UTR的单核苷酸多态性(SNP)位点基因分布频率,同时分析胃癌患者LAIR1基因3’UTR的多态性与肿瘤分化程度的关系。结果胃癌组患者rs199714928位点G/A基因型及等位基因A分布频率均明显低于对照组(均P<0.05),G/G基因型及等位基因G分布频率均明显高于对照组(均P<0.05)。胃癌组rs1054033与rs6509867位点均存在完全单倍型连锁现象,而对照组有16例存在不完全单倍型连锁现象(P<0.05)。胃癌低分化患者rs3826753/rs3826754位点A/A基因型及等位基因A分布频率均明显低于中高分化者(均P<0.05),等位基因G分布频率明显高于中高分化者(P<0.05);不同性别、肿瘤类型、TNM分期、淋巴结转移阳性率患者LAIR1基因3’UTR的SNP位点基因分布频率比较,差异均无统计学意义(均P>0.05)。结论 LAIR1基因3’UTR的rs199714928位点多态性可能是胃癌发生的易感因素;rs1054033与rs6509867位点存在不完全单倍型连锁(T-G单倍型)可能是一种保护机体抵抗胃癌发生的有利因素。

Objective To investigate the association of the human inhibitory leukocyte-associated Ig-like receptor 1(LAIR1)polymorphisms in the 3忆-untranslated region(3'UTR)with the susceptibility of gastric cancer.Methods One hundred and twenty one patients with gastric cancer and 126 age and gender-matched patients with chronic gastritis patients(control group)were recruited.The 3'UTR polymorphisms of LAIR1 were detected by sanger sequencing.The distribution frequency were analyzed to evaluate the susceptibility of gastric cancer and the correlation with the degree of cancer differentiation.Results The frequency of rs199714928 G/G genotype and allele G was significantly higher in the gastric cancer patients(P<0.05),compared with the controls.Rs1054033 was completely linked to rs6509867 in gastric cancer patients,while there was 16 controls who were not haplotype-linked(T-G haplotype)(P<0.05).In addition,the frequency of rs3826753/rs3826754 genotype A/A and allele A in poorly differentiated gastric cancer patients was significantly lower than that of patients with moderate and high differentiation(P<0.05).Conclusion The study indicated that the rs199714928 polymorphismin the 3忆UTR of the LAIR1 gene may be associated with the susceptibility of gastric cancer.The incomplete haplotype linkage between rs1054033 and rs6509867(T-G haplotype)might protect individuals fromthe development of gastric cancer.