TNF-α、IL-1β与脑出血患者神经细胞凋亡关系研究

Relationship between inflammatory factors TNF-α and IL-1β and neuronal apoptosis in patients with intracerebral hemorrhage

目的探究炎性因子肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)与脑出血(ICH)患者神经细胞凋亡之间的关系。方法选取本院2015年3月至2016年9月收治的85例ICH患者。免疫组化法、RT-PCR法及Western blot法检测在不同时间段脑组织中TNF-α、IL-1β的表达,采用TUNEL法检测在不同时间段皮质脑组织中神经元细胞的凋亡。分析炎性因子表达与神经元细胞凋亡的相关性。结果与对照组阳性细胞表达数相对比, TNF-α和IL-1β阳性细胞数各实验组中的表达均显著升高(P<0.001),ICH后TNF-α在13～48h表达达到高峰,IL-1β在24h内表达达到高峰。各实验组凋亡细胞数均明显高于对照组凋亡细胞数,对比差异显著(P<0.001),13～48h为细胞凋亡高峰期。TNF-α、IL-1β阳性细胞数与神经元细胞凋亡数均呈明显的正相关性(R^2=0.5731和0.6914,P<0.0001)。结论 ICH患者的神经元细胞凋亡与炎性因子TNF-α、IL-1β的表达呈一致性。

Objective To evaluate the relationship between expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and apoptosis in patients with intracerebral hemorrhage(ICH).Method 85 patients with ICH were selected as the experiment group,Immunohistochemistry,RT-PCR and Western blot were used to detect the expression of TNF-α,IL-1βin brain tissues at different time periods,TUNEL was used to detect the apoptosis of neurons.The correlation of TNF-α,IL-1βexpression and apoptosis was analyzed using pearson test.Results The number of TNF-α,IL-1βpositive cells in each experimental group was significantly higher than that in the control group,which was statistically significant(all P<0.001).After ICH,the expression of TNF-αpeaked at 13-48h,and expression of IL-1βreached its peak in 24h.The number of apoptotic cells in each experimental group was significantly higher than that in the control group(P<0.001),and 13-48h was the peak of apoptosis.The number of TNF-α,IL-1βpositive cells were positively correlated with the number of apoptosis of neurons.Conclusion The number of apoptosis of neuronal cells in patients with ICH was consistent with the expression of TNF-αand IL-1β.