摘要
AIM: To study the impact of association between cytomegalovirus(CMV) pathogenesis with dendritic cell(DC) maturation and function was evaluated in CMV reactivated liver transplanted patients in comparing with non-reactivated ones, and healthy controls. METHODS: Monocyte derived dendritic cells(Mo DCs) was generated from collected ethylenediaminetetraacetic acid-treated blood samples from patient groups and controls. In these groups, expression rates and mean fluorescent intensity of DC markers were evaluated using flowcytometry technique. Secretion of cytokines including: interleukin(IL)-6, IL-12 and IL-23 were determined using enzyme-linked immunosorbent assay methods. The gene expression of toll-like receptor 2(TLR2), TLR4 and IL-23 were analyzed using in-house real-time polymerase chain reaction protocols.RESULTS: Results have been shown significant decreases in: Expression rates of Mo DC markers including CD83, CD1 a and human leukocyte antigen DR(HLA-DR), the mean fluorescence intensitys for CD1 a and HLA-DR, and secretion of IL-12 in CMV reactivated comparedwith non-reactivated liver transplanted patients. On the other hand, significant increases have been shown in the secretions of IL-6 and IL-23 and gene expression levels of TLR2, TLR4 and IL-23 from Mo DCs in CMV reactivated compared with non-reactivated liver transplanted recipients. CONCLUSION: DC functional defects in CMV reactivated recipients, such as decrease in expression of DC maturation markers, increase in secretion of proinflammatory cytokines, and TLRs can emphasize on the importance of CMV infectivity in development of liver rejection in transplanted patients.
AIM: To study the impact of association between cytomegalovirus(CMV) pathogenesis with dendritic cell(DC) maturation and function was evaluated in CMV reactivated liver transplanted patients in comparing with non-reactivated ones, and healthy controls. METHODS: Monocyte derived dendritic cells(Mo DCs) was generated from collected ethylenediaminetetraacetic acid-treated blood samples from patient groups and controls. In these groups, expression rates and mean fluorescent intensity of DC markers were evaluated using flowcytometry technique. Secretion of cytokines including: interleukin(IL)-6, IL-12 and IL-23 were determined using enzyme-linked immunosorbent assay methods. The gene expression of toll-like receptor 2(TLR2), TLR4 and IL-23 were analyzed using in-house real-time polymerase chain reaction protocols.RESULTS: Results have been shown significant decreases in: Expression rates of Mo DC markers including CD83, CD1 a and human leukocyte antigen DR(HLA-DR), the mean fluorescence intensitys for CD1 a and HLA-DR, and secretion of IL-12 in CMV reactivated comparedwith non-reactivated liver transplanted patients. On the other hand, significant increases have been shown in the secretions of IL-6 and IL-23 and gene expression levels of TLR2, TLR4 and IL-23 from Mo DCs in CMV reactivated compared with non-reactivated liver transplanted recipients. CONCLUSION: DC functional defects in CMV reactivated recipients, such as decrease in expression of DC maturation markers, increase in secretion of proinflammatory cytokines, and TLRs can emphasize on the importance of CMV infectivity in development of liver rejection in transplanted patients.
基金
financially supported using a grant from Iran National Science Foundation
Transplant Research Center of Namazi Hospital,Shiraz,Iran for Lab support
