摘要
建立了同时筛查确认化妆品中喹诺酮类、糖皮质激素类、硝基咪唑类、四环素类、大环内酯类、磺胺类、头孢类和性激素8类112种禁用药物的高效液相色谱-质谱联用分析方法。试样采用0. 2%甲酸乙腈作为提取液,通过Qu ECh ERS净化后过滤膜上机检测。以0. 2%甲酸水-0. 2%甲酸乙腈溶液为流动相梯度洗脱,C18色谱柱分离,质谱动态多反应监测(DMRM)模式进行分析。结果表明,112种药物的定量下限为2. 0~10. 0μg/kg;加标回收率为82. 0%~108%;相对标准偏差(RSD)为2. 0%~7. 9%。该方法操作简单、快速、高效,适用于化妆品中上述几类禁用药物的快速筛查和测定。
An analytical method was established for the simultaneous screening and determination of 112 prohibited drugs,including quinolones,glucocorticoids,nitroimidazoles,tetracyclines,macrolides,sulfonamides,cephems and androgens in cosmetics by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The sample was extracted with 0.2%formic acid-acetonitrile,and then purified by QuEChERS method.The extracts were separated on an Agilent ZorbaxEclipsePlus C 18(3.0 mm×150 mm,1.8μm)column with 0.2%formic acid-acetonitrile solution containing 0.2%formic acid as mobile phase by gradient elution,and analyzed by LC-MS/MS under dynamic multiple reaction monitoring(DMRM)mode.Results showed that the limits of quantitation(LOQ,S/N=10)for 112 target compounds in cosmetics were in the range of 2.0-10.0μg/kg.The mean recoveries for 112 prohibited drugs were in the range of 82.0%-108%with their RSDs of 2.0%-7.9%.The method has the advantages of simplicity,rapidness and high efficiency,and could be applied in the screening and confirmation on 112 prohibited drugs in cosmetics in routine analysis.
作者
陈少波
蒲云月
王海瑞
徐振东
CHEN Shao-bo;PU Yun-yue;WANG Hai-rui;XU Zhen-dong(Inspection and Quarantine Technical Center,Suzhou 215104,China;Suzhou World Standard Testing Technology Co.,Ltd.,Suzhou 215104,China;Suzhou Huabo Daily Chemicals Testing& Service Co.,Ltd.,Suzhou 215104,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2018年第11期1302-1310,共9页
Journal of Instrumental Analysis
基金
国家质量监督检验检疫总局科技计划项目(2016IK142)
江苏检验检疫局科技计划项目(2017KJ48)