乳腺癌中Wnt/β-catenin信号通路相关蛋白的表达及意义

Expression of Wnt/β-catenin signaling pathway-related proteins in breast cancer and its clinical significance

目的:本研究旨在探讨乳腺癌组织和细胞中Wnt/β-catenin信号通路相关蛋白Wnt-1和β-catenin的表达及意义。方法:选取我院于2015年1月~2017年1月间收治的150例乳腺癌患者作为研究对象。150例乳腺癌的肿瘤组织样本(实验组)来源于手术切除的、临床病理资料保存完整的乳腺癌石蜡切块;相应的临近非肿瘤组织样本(对照组)取自距离肿瘤组织0.5~1 cm的上述乳腺癌患者相应癌旁组织。采用real-tme PCR和Western blot法检测Wnt-1和β-catenin的表达情况。将乳腺癌细胞MCF-7分为3组:阴性对照组(MCF-7细胞)、激动剂组[MCF-7细胞+Wnt3a(1 mg/L)]和拮抗剂组[MCF-7细胞+DKK1(16μmol/L)]。采用real-time和Western blot检测Wnt-1和β-catenin表达情况。结果:与对照组相比,实验组患者Wnt-1和β-catenin的mRNA和蛋白表达量均升高(P<0.05)。实验组患者肿瘤组织的Wnt-1表达阳性率和β-catenin表达阳性率均高于对照组(P<0.05)。Wnt-1表达与乳腺癌患者肿瘤转移(x^2=5.352,P=0.021)、肿瘤分期(x^2=9.412,P=0.002)及肿瘤直径(x^2=9.412,P=0.002)密切相关(P<0.05)。β-catenin表达与乳腺癌患者肿瘤转移(x^2=9.851,P=0.002)、肿瘤分期(x^2=5.661,P=0.017)密切相关(P<0.05)。与对照组相比,激动剂组Wnt-1和β-catenin mRNA和蛋白表达量升高(P<0.05);与对照组相比,拮抗剂组Wnt-1和β-catenin mRNA和蛋白表达量降低(P<0.05)。结论:乳腺癌中Wnt/β-catenin信号通路相关蛋白Wnt-1和β-catenin表达上调,且其表达与肿瘤恶性状态密切相关。

AIM:To evaluate the expression of Wnt/β-catenin signaling pathway-related proteins in breast cancer and the significance.METHODS:The patients with breast cancer(n=150)in our hospital from January 2015 to January 2017 were selected as study object.The tumor tissue samples of these patients were obtained from paraffin section of breast cancer by surgical resection with complete clinicopathological data.The corresponding paracancerous tissue sam-ples were taken from the non-tumor tissue samples from the above breast cancer patients,which were 0.5~1 cm away from the tumor tissue.The methods of real-time PCR and Western blot were performed to examine the expression of Wnt-1 andβ-catenin at mRNA and protein levels.Human breat cancer MCF-7 cells were divided into 3 groups:control group(MCF-7 cells without treatment),agonist group[MCF-7 cells+Wnt3a(1 mg/L)]and antagonit group[MCF-7 cells+DKK1(16μmol/L)].The expression of Wnt-1 andβ-catenin at mRNA and protein levels was detected by real-time PCR and Western blot.RESULTS:Compared with the paracancerous tissues,the expression levels of Wnt-1 andβ-catenin were higher in tumor tissues at mRNA and proteins levels(P<0.05).Notably,the positive expression rates of Wnt-1 andβ-catenin were significantly higher in tumor tissues than that in the paracancerous tissues.Furthermore,Wnt-1 expression was associated with tumor metastasis(2=5.352,P=0.021),tumor stage(2=9.412,P=0.002)and tumor size(2=9.412,P=0.002).In addition,β-catenin expression was also associated with tumor metastasis(2=9.851,P=0.002)and tumor stage(2=5.661,P=0.017).Compared with control group,the expression of Wnt-1 andβ-catenin at mRNA and protein levels in agonist group was increased(P<0.05),while that in antagonist group was decreased(P<0.05).CONCLUSION:The expression levels of Wnt-1 andβ-catenin related with Wnt/β-catenin signaling pathway are increased in the breast cancer,which are closely related to the malignant state of the tumor.