两株长喙壳属真菌挥发性香气成分分析

The Aroma Components Analysis of Two Ceratocystis Strains

长喙壳属真菌具有产香特性,本文比较了长喙壳菌株PP1-1PG、WYB的菌落形态、r DNA-ITS序列等特征,并利用气相色谱-质谱(GC-MS)联用技术分析其有机化合物组成。结果表明,菌株在PSA培养基上菌丝不发达,为有隔菌丝。菌株PP1-1PG在ITS基因序列系统进化树上与Ceratocystis fimbriata聚为一支,菌株WYB与Ceratocystis paradoxa聚为一组。GC-MS分析表明,两个菌株产生的挥发性香气物质组成主要为乙酸乙酯、乙酸异丁酯、苯乙烯等,其中乙酸乙酯、乙酸异丁酯相对含量最高,二者之和在两个菌株中分别达到45. 85%与61. 02%。本研究首次在菌株PP1-1PG中检测到重要的香料物质苯甲醛(安息香醛),为开发利用苯甲醛提供了参考依据。

The genus Ceratocystis produces a range of flavoring compounds,in this study,strains PP1-1PG and WYB were compared on the basis of characteristics of colony morphology and ITS sequences.The volatile components were analyzed by gas chromatography-mass spectrometry(GC-MS).Septate mycelia were observed on PSA substrates for both strains.Based on the ITS phylogeny,strain PP1-1PG was clustered with Ceratocystis fimbriata-forming a distinct clade while strain WYB was clustered with Ceratocystis paradoxa.Analyzing the volatile components by GC-MS,it is known that the most materials produced by two strains were ethyl acetate,isobutyl acetate and styrene,which the relative contents of ethyl acetate and isobutyl acetate were 45.85%and 61.02%in two strains.Benzaldehyde,a widely used flavour substance,was detected from strain PP1-1PG for the first time,providing chemical basis and foundation for the comprehensive development and the search for this substance.