桑枝中桑皮苷A的提取工艺优化及其含量测定

Optimization on Extraction Technology and Determination of Mulberroside A in Morus alba( L. ) by UPLC

本文采用响应面法优化桑枝中桑皮苷A的超声提取工艺。在单因素试验的基础上,以桑枝中桑皮苷A为响应值,考察了甲醇比例、溶剂体积、超声时间及超声温度对桑皮苷A含量测定的影响。研究表明,桑枝中桑皮苷A的最佳提取工艺条件为:60%甲醇水溶液,溶剂体积55 mL,超声时间40 min,超声温度35℃。在该条件下,桑枝中桑皮苷A质量分数为7. 63 mg/g,与预测值7. 64 mg/g接近。桑皮苷A在0. 012 8~0. 064 mg/mL范围呈良好的线性关系(r=0. 999 3),9批桑枝药材中桑皮苷A含量范围为4. 97~13. 14 mg/g。结果表明根据Box-Behnken模型、采用响应面分析法得到的桑枝中桑皮苷A提取优化工艺准确可靠,可用于检测桑枝中桑皮苷A的含量。

Response Surface Methodology(RSM)was applied to optimize of ultrasonic extracting technique for mulberroside A in Morus alba(L.).On the basis of a single factor test,the effects of the proportion of methanol concentration,the volume of solvent,the extraction time and the extraction temperature on the determination of the content of the mulberroside A in the Morus alba(L.)were investigated.On the basis of sigle-factor experiments,the optimum extraction process of mulberroside A in Morus alba(L.)is methanol concentration(60%),solvent volume(55 mL),the extraction time(40 min)and the extraction temperature(35℃).Under the optimal extraction condition,the mulberroside A productivity was 7.63 mg/g,which was in consistent with the predicted value of 7.64 mg/g.The calibration curve was in good linearity within the range from 0.0128 to 0.064 mg/mL(r=0.9993).The content of mulberroside A in Morus alba(L.)in defferent areas was 4.97~13.14 mg/g.The preferred process of optimizing the extraction process of mulberroside A in Morus alba(L.)with response surface analysis is stable and feasible,and can be used for the determination of mulberroside A in Morus alba(L.).