miR-27a、miR-27b和miR-378在不同猪种皮下脂肪中的差异表达研究

Differential Expression of miR-27a, miR-27b and miR-378 in Subcutaneous Adipose Tissue of Different Pig Breeds

为研究miRNA表达量与猪胴体及肉质性状的相关性,实验采用QRT-PCR技术检测了引进猪种大约克夏猪和6个四川省地方猪种背部皮下脂肪中miR-27a、miR-27b、miR-378的差异表达情况。结果表明:miR-27a、miR-27b、miR-378在大约克夏猪背部皮下脂肪中的表达水平均显著高于6个地方猪种(P<0.05);地方猪种中,3种miRNA表达水平均表现为在雅南猪中最高;3种miRNA在背部皮下脂肪中相对表达量均与瘦肉率、多不饱和脂肪酸含量呈极显著正相关(P<0.01)。综上表明,miR-27a、miR-27b和miR-378均可抑制猪脂肪沉积,提高胴体性能,但对猪肉质具有负调控作用。

The expression levels of miR-27a,miR-27b and miR-378 were investigated in back subcutaneous adipose tissues of Yorkshire and 6 indigenous pigs using quantitative real-time polymerase chain reaction(QRT-PCR).The results showed that the expression levels of the 3 miRNAs of Yorkshire pigs were the significantly higher than that of indigenous pigs(P<0.01).Furthermore,the expression levels of the 3 miRNAs of Yanan pigs were highest among 6 indigenous pigs.Relevance analysis showed that the expression levels of miR-27a,miR-27b and miR-378 had a significantly positive correlation with lean meat percentage and polyunsaturated fatty acids(PUFAs)percentage(P<0.01).These findings indicated that miR-27a,miR-27b and miR-378 may inhibit adipose deposition and improve carcass performance,but have negative regulatory effect on meat quality.