五味子酚对心肌成纤维细胞增殖和转化生长因子β_1及Ⅰ、Ⅲ型胶原蛋白表达的影响

Effect of Schisanhenol on proliferation of cardiac fibroblasts and potential mechanisms

目的研究五味子酚(Sal)对血管紧张素Ⅱ(AngⅡ)诱导的心肌成纤维细胞(CFb)增殖及转化生长因子β_1(TGF-β_1)和Ⅰ、Ⅲ型胶原蛋白表达的影响,探讨Sal抗心肌纤维化的可能机制。方法采用酶消化法结合差速贴壁法获得纯化的CFb;利用免疫荧光法鉴定CFb;采用MTT法检测Sal对CFb增殖的影响;采用Western blot检测CFb的TGF-β_1及Ⅰ、Ⅲ型胶原蛋白表达水平。结果在一定浓度范围内,Sal能抑制AngⅡ诱导的CFb增殖且具有剂量依赖性(P <0.05);Sal能抑制TGF-β_1及Ⅰ、Ⅲ型胶原蛋白表达,与AngⅡ组比较差异有统计学意义(P<0.05),且具有剂量依赖性。结论 Sal能抑制CFb增殖及降低Ⅰ、Ⅲ型胶原蛋白表达,其机制可能与下调TGF-β_1蛋白表达有关。

Objective To investigate the effect of Schisanhenol(Sal)on proliferation of cardiac fibroblasts(CFb)and potential mechanisms.Methods Purified CFb were obtained by standard methods(digestive enzyme and differential adhesion).CFb were induced by angiotensin II(Ang II)and identified by immunofluorescence.Cellular proliferation of CFb was measured by methyl thiazolyl tetrazolium(MTT)assay.Levels of TGF-β1,collagen I and collagen III proteins were examined by Western blotting.Results Sal inhibited proliferation of CFb in a dose-dependent manner(P<0.05).Expression of collage I,collagen III,and TGF-β1 induced by Ang II was downregulated by treatment of Sal in a dose-dependent manner(P<0.05).Conclusion Sal inhibits cellular proliferation and expression of collagen through inhibition of the TGF-β1.