GRHPR在肝细胞癌组织和细胞中的表达及意义

Expression and significance of GRHPR in hepatocellular carcinoma tissue and cells

目的研究乙醛酸还原酶/羟基丙酮酸还原酶(GRHPR)在肝细胞癌(HCC)中的表达,与肝癌细胞增殖相关性,及其对HCC患者预后分析。方法采用Western blot检测GRHPR在8例肝癌及癌旁组织标本和肝癌细胞株的表达情况;采用免疫组织化学方法检测120例HCC及相应癌旁组织微阵列中GRHPR和增殖指标Ki-67的表达情况,并结合肝癌临床病理资料分析GRHPR与HCC患者的年龄、性别、肿瘤分级、大小和转移及血清甲胎蛋白(AFP)等因素之间的关系,并对上述病例进行随访,应用Kaplan-Meier法分析HCC患者生存率。结果采用Western blot检测发现GRHPR在新鲜活体肿瘤组织中表达比在癌旁组织中表达减少,在肿瘤细胞株中表达也减少;免疫组织化学检测结果显示GRHPR表达与Ki-67呈负相关性(r=-0.870,P<0.05);在癌和相应癌旁组织中GRHPR阴性表达的患者生存率低于GRHPR阳性患者(P<0.05)。Kaplan-Meier分析结果显示GRHPR在HCC中的阳性表达与患者总生存率的增加显著相关(P<0.01)。结论GRHPR在癌和癌旁组织缺失提示GRHPR可能是肝癌发生、发展过程中的保护因素。

Objective To investigate the expression of glyoxylate reductase/hydroxypyruvate reductase(GRHPR)in hepatocellular carcinoma(HCC)and its correlation with HCC proliferation and its prognosis analysis in HCC patients.Methods The expression level of GRHPR in 8 cases of tissue sample and HCC cell line was detected by adopting the Western blot;the immunohistochemistry was utilized to examine the expression levels of GRHPR and Ki-67 in 120 cases of HCC and corresponding paracancerous tissue microarray.The correlation between GRHPR with the factors of age,sex,tumor grade,size,metastasis and serum AFP was analyzed by combining with the HCC clinicopathological data.The above cases were followed up.The survival rate of HCC patients was analyzed by using the Kaplan Meier method.Results The Western blot detection found that the GRHPR expression in fresh in vivo tumor tissue was decreased compared with the non-cancerous tissue;the immunohistochemical detection results showed that the GRHPR expression was negatively correlated with Ki-67(r=-0.870,P<0.05);the survival rate in the patients with GRHPR expression negative in cancer and corresponding paracancerous tissue was lower than that in the patients with GRHPR positive(P<0.05).The Kaplan-Meier analysis results showed that the GRHPR positive expression in HCC was significantly correlated with the total survival rate of the patients(P<0.01).Conclusion The deficiency of GRHPR in cancer and paracancerous tissue prompts that GRHPR may be a protective factor of the occurrence and development process of HCC.