HCT值实时修正全血CRP测定结果的评估

Evaluation on the real-time correction of whole blood CRP determination using HCT value

目的利用血细胞比容(HCT)值对全血C反应蛋白(CRP)测定结果进行实时修正,以保证结果的准确性。方法收集217例患者的血常规标本,其中37例HCT<35%、128例HCT为35%～45%,52例HCT>45%。评估XN 2800血常规CRP流水线修正前后CRP结果的可比性。结果对于HCT<35%的37例标本,未修正HCT的CRP与BNⅡ特定蛋白分析仪检测结果间平均偏差为11.77 mg/L,偏差大小与HCT值降低呈负相关,修正后CRP检测结果平均偏差为2.75 mg/L。对于HCT为35%～45%的128例标本,未修正HCT的CRP与BNⅡ特定蛋白分析仪检测结果间平均偏差为2.79mg/L,修正后CRP检测结果平均偏差为2.77mg/L。对于HCT>45%的52例标本,未修正HCT的CRP与BNⅡ特定蛋白分析仪检测结果间平均偏差为-11.84 mg/L,偏差大小与HCT值升高呈正相关,修正后CRP检测结果平均偏差为-1.35 mg/L。结论对HCT<35%和>45%的标本,宜采用HCT值实时修正CRP结果,使检测结果更为准确。

Objective To evaluate the real-time correction of whole blood C-reactive protein(CRP)determination using hematocrit(HCT)value in order to ensure the accuracy of determination results.Methods A total of 217 blood samples were collected.HCT<35%was found in 37 cases,HCT 35%-45%in 128 cases,and HCT>45%in 52 cases.The comparability of CRP levels before and after correcting in XN 2800 was evaluated.Results The mean deviation between uncorrected level and target CRP level by BNⅡspecific protein analyzer was 11.77 mg/L in 37 cases with HCT<35%,and the deviation was negatively correlated with the decrease of HCT,while the mean deviation between corrected and target CRP levels was 2.75 mg/L.In 128 cases with HCT 35%-45%,the mean deviation between uncorrected and target CRP levels was 2.79 mg/L,while the mean deviation between corrected and target CRP levels was 2.77 mg/L.In 52 cases with HCT>45%,the mean deviation between uncorrected and target CRP levels was-11.84 mg/L,and the deviation was positively correlated with the increase of HCT,while the mean deviation between corrected and target CRP levels was-1.35 mg/L.Conclusions As to blood samples with HCT<35%and HCT>45%,real-time correction of whole CRP value determination using HCT value can make the determination results more accurate.