常温大气压等离子体对番茄种带细菌的灭菌效果

Sterilization effects of atmospheric pressure room-temperatureplasma treatment on tomato seeds

为了明确常温大气压等离子体(atmospheric pressure room-temperature plasma,APRTP)在番茄种子消毒中的应用效果,以‘中杂302’种子为材料,采用平板培养计数和16S rRNA拷贝数分析法,测定了APRTP处理对种子表面和内部细菌的灭菌效果。结果表明,APRTP处理对种带细菌的灭菌效果显著,在5～30 min处理时间内,灭菌率随处理时间延长逐渐升高,处理25 min对种子表面和内部细菌的灭菌率达99%以上;番茄种子接种细菌性溃疡病致病菌—密执安棒杆菌密执安亚种(Cmm)后,采用APRTP处理25 min可有效杀灭99.84%的种带Cmm菌;种子活力检测表明,APRTP处理可提高番茄种子活力,加快种子萌发速率,APRTP处理过的种子播种后,对幼苗早期生长不会造成伤害。综上,APRTP处理可显著杀灭番茄种带细菌,提高种子活力、促进种子萌发,是一种安全、高效的番茄种子处理方法。

Cultivar‘Zhongza 302’seeds were used as materials to evaluate the sterilization effects of atmospheric pressure room-temperature plasma(APRTP)on seeds during tomato seed treatment.The bacteria on seed surface and in the seed were determined by analyzing the number of culturable bacteria and the 16S rRNA copies.The results showed that the sterilization effects of APRTP on tomato seed-borne bacteria were significant.The sterilization rate increased with the APRTP processing time increased from 5 to 30 minutes,and the sterilization rate could reach to more than 99%after APRTP treatment for 25 min for both surface bacteria and internal bacteria in the seed.APRTP was used to eliminate Clavibacter michiganensis subsp.michiganensis(Cmm)inoculated to tomato seeds,the sterilization rate to Cmm could reach to 99.84%when samples were processed for 25 min.Furthermore,detection of seed vigor showed that APRTP treatment could increase the seed vigor,and thereby improve the germination potential and accelerate seed germination.After sowing,the APRTP-treated seeds showed no significant damages for the seedlings growth and development.Taken together,APRTP treatment showed significant sterilization effects on seed-borne bacteria and increased the seed vigor,indicating that it was an efficient and safe method for tomato seed treatment.