人口腔鳞状细胞癌Tca8113细胞过表达WWOX基因的基因表达谱分析及WWOX基因的抑癌作用机制

Analysis on gene profile of over-expression WWOX gene in human oral squamous cell carcinoma Tca8113 cells and tumor inhibition mechanism of WWOX gene

目的:分析人口腔鳞状细胞癌(OSCC)Tca8113细胞过表达包含WW域的氧化还原酶(WWOX)基因的基因表达谱变化,探讨WWOX基因的抑癌作用机制。方法:Tca8113细胞分为WWOX基因过表达组和对照组。采用携带WWOX基因的pGV287-LV-WWOX慢病毒颗粒和携带空载体的pGV287-LV慢病毒颗粒分别感染2组Tca8113细胞。采用实时荧光定量PCR (qRT-PCR)和Western blotting法检测2组Tca8113细胞中WWOX mRNA和蛋白表达情况。采用基因芯片技术筛选差异表达基因并进行生物学分析。采用定量PCR法检测丝裂原活化蛋白激酶(MAPK)信号通路的差异表达基因的mRNA相对表达水平。结果:慢病毒感染后,WWOX基因过表达组Tca8113细胞中WWOX mRNA相对表达水平较对照组明显升高(P<0.05),WWOX基因过表达组Tca8113细胞中WWOX蛋白表达水平升高。基因芯片筛选出差异倍数(FC)1.5倍以上的基因共347个,其中表达上调171个,表达下调176个。生物信息学分析显示这些基因分布于癌症、p53信号、MAPK信号和白细胞介素2 (IL-2)等多条信号传导通路。WWOX基因过表达组MAPK信号通路中的丝裂原活化蛋白激酶2 (MAP2K)、孤核受体4A1 (NR4A1)、双特异性磷酸酶5 (DUSP5)和双特异性磷酸酶6 (DUSP6)mRNA相对表达水平较对照组明显升高(P<0.05),而成纤维生长因子受体2 (FGFR2)mRNA相对表达水平较对照组明显降低(P<0.05)。结论:WWOX基因过表达导致Tca8113细胞的基因表达谱发生变化,WWOX基因的抑癌作用机制与调节MAPK信号途径的多个基因表达有关联。

Objective:To analyze the changes of gene profile of over-expression WWOX gene in the human oral squamous cell carcinoma(OSCC)Tca8113 cells,and to explore the tumor inhibition mechanism of WW OX gene.Methods:The Tca8113 cells were divided into WWOX over-expression group and control group.In WWOX over-expression group,the lentiviral plasmid carrying the full-length cDNA fragment of WWOX gene was infected into the Tca8113 cells,while in control group,the lentiviral plasmid carrying the empty pGV287-LV vector was infected into the Tca8113 cells.The quantitative real time PCR(qRT-PCR)and Western blotting methods were used to detect the expressions of WWOX mRNA and protein.The gene chip technique was used to screen the differientially expressed genes and biological analysis was performed.The quantitative PCR was used to detect the relative mRNA expression levels of differientially expressed genes involving in the mitogen-activated protein kinase(MAPK)signaling pathway.Results:After infection,the relative mRNA expression level of WWOX gene in the Tca8113 cells in WWOX over-expression group was higher than that in control group(P<0.05),and the expression of WWOX protein in the Tca8113 cells in WWOX over-expression group was higher.A total of 347 differientially expressed genes with a 1.5-fold-change(FC)were screened by gene chip,in which 171 genes showed up-regulated expression,and 176 genes showed down-regulated expression.The Bioinformation analysis results showed that these different genes distributed in several pathways,which were related to cancer,p53,MAPK,and interleukin-2(IL-2)pathways and so on.Compared with control group,the relative expression levels of MAP2K,NR4A1,DUSP5,and DUSP6 mRNA in MAPK signaling pathway in WWOX over-expression group were increased(P<0.05),and the relative expression level of fibroblast growth factor receptor 2(FGFR2)mRNA was decresed(P<0.05).Conclusion:The over-expression of WWOX gene in the Tca8113 cells can induce the changes of gene profile of Tca8113 cells.The tumor inhibition mechanism of WWOX gene may be related to regulating the expressions of some genes involving in MAPK signaling pathway.