丝素蛋白/聚乙烯醇复合膜的制备及其表征

Preparation and characterization of silk fibroin /polyvinyl alcohol composite membrane

为探讨离子液体共溶法对所制备丝素蛋白复合膜的结构与性能的影响,用离子液体1-烯丙基-3-甲基咪唑氯盐([Amim]Cl)分别溶解脱胶蚕丝和聚乙烯醇粉末,然后将2个溶解体系按不同质量比共混后倒入成膜器,经甲醇浸泡去除[Amim]Cl后自然风干成膜。借助紫外分光光度计、X射线衍射仪、扫描电子显微镜、光学接触角仪、紫外灯耐气候试验箱对复合膜的性能进行测试与表征。结果表明:[Amim]Cl共溶法成膜可提高丝素蛋白与聚乙烯醇组分的相容性,成膜过程中丝素蛋白组分向膜的表面迁移,并主要以Silk II构象存在;随着复合体系中丝素蛋白配比的增加,复合膜表面粗糙度增加,膜的亲水性能提升,同时还加速了复合膜的光老化进程。

In order to discuss the influence of co-dissolution processing in ionic liquid on structure and properties of silk fibroin composite membrane,the dugummed silk fiber and polyvinyl alcohol powder were dissolved by ionic liquid 1-allyl-3-methyl imidazolyl chloride([Amim]Cl),respectively,and then the two dissolving systems were mixed according to different mass proportions and poured into a film former.After being soaked in methanol,[Amim]Cl was removed and naturally air-dried to form membranes.The composite membranes were tested and characterized by ultraviolet spectrophotometer,X-ray diffractometer,scanning electron microscope,optical contact angle meter and ultraviolet light resistant test box.The results show that the compatibility of silk fibroin and polyvinyl alcohol components can be improved by the[Amim]Cl co-dissolution method,the silk fibroin component during film formation migrates to the surface of the membrane,and its main conformation is the silk II structure.In addition,the surface roughness of the composite membrane increases with the increasing of the ratio of silk fibroin in the composite system,leading to the improvement of the hydrophilicity of the composite membrane.At the same time,the increasing of the ratio of silk fibroin will accelerate the photo-aging process of the composite membrane.