礁膜室内规模化人工育苗的研究

Indoor Artificial Seedling of Green Seaweed Monostroma nitidium on a Large Scale

本文研究了礁膜人工育苗全过程,包括成熟礁膜的配子日放散规律,合子附着的最适附着基及黑暗时间,合子培养过程中杂藻硅藻的清除。结果显示,成熟礁膜可在24h中任何时段放散配子;合子附着的最适附着基为PVC塑料板,且在黑暗处理8~12h时合子附着最好;用0.5~3mg/L二氧化锗处理11d能有效去除合子培养过程中滋生的硅藻。根据以上育苗技术参数,2016年4月至10月于室内进行了规模化人工育苗试验,初步建立了礁膜规模化人工育苗技术流程。本次试验共用0.9kg新鲜藻体(含水量约60%),共放散4×1010个配子,平均每克藻体放散配子量高达4.4×108个;共采PVC苗板200张(规格20cm×10cm×1mm),平均每张PVC苗板附有4×107个合子;合子经培养生长发育为成熟孢子囊并释放游孢子,采用苗绳(直径=1cm)附着游孢子,培育15d后幼苗长度可达1cm。

In this paper,artificial seedling of green seaweed Monostroma nitidium on a large scale was studied,including regularity of gamete releasing from blades of the seaweed,the optimal attachment and darkness period for adherence of zygotes,and removal of diatoms in the culture of zygotes.It was fond that the seaweed released gametes at any time of a day.A PVC plastic plate was the optimal attaching substratum of the zygotes with good settlement rate at darkness of 8-12 h.The high removal efficiency of diatoms was observed at GeO 2 concentration of 0.5-3 mg/L for 11 days.From the above technical parameters,a large-scale artificial seedling test was conducted in an indoor from April to October,2016,and the technological process of artificial seedling of the seaweed was established.About 4×10 10 gametes were released from 0.9 kg fresh thallus,with average gametes releasing rate of about 4.4×10 8 gametes/g,and total about 200 PVC plastic plates(20 cm×10 cm×1 mm)were used for breeding,with average zygotes attaching rate of about 4×10 7 zygotes/plate.The zygote grew and developed into mature sporangia after breeding,and sporangia released spores.The ropes(D=1 cm)were used for seedling,with the seedling length of 1cm in 15 day cultivation.