摘要
试验基于NRC-2001模型预测不同玉米副产品代谢蛋白产量,利用光谱技术分析不同玉米副产品的分子功能团特征及二者之间的相关性并建立回归方程。采用NRC-2001模型对奶牛饲料蛋白营养价值进行评价,利用傅立叶变换红外光谱(Fourier transform infrared,FTIR)技术收集光谱数据并分析。结果显示,利用NRC-2001模型预测不同玉米副产品代谢蛋白特征参数具有显著差异(P<0.05)。其中,小肠中真可吸收代谢蛋白总量在玉米蛋白粉中有最高值,其次是玉米胚芽饼、玉米胚芽、玉米皮及玉米籽粒。饲料产奶价值(FMV)存在相同趋势。在光谱结构分析中,饲料的分子功能团特征参数存在显著差异(P<0.05),蛋白代谢预测产量与分子功能团特征参数之间存在相关性,建立的回归模型拟合度较好。其中,玉米籽粒中酰胺Ⅰ带与纤维性碳水化合物的面积比值(AmideⅠ_CELC)显著高于其他饲料原料(P<0.05),玉米胚芽的酰胺Ⅰ带与结构性碳水化合物的面积比值(AmideⅠ_SCHO)和AmideⅠ_CELC比值均较低(P<0.05),而玉米皮的蛋白质酰胺Ⅱ带与结构性碳水化合物(SCHO)和纤维性碳水化合物(CELC)之间的光谱面积比值均显著低于其他饲料比值(P<0.05),玉米胚芽饼的蛋白质酰胺Ⅱ带与总碳水化合物之间峰面积比值(AmideⅡ_TNSCHO)显著高于其他饲料原料(P<0.05)。在不同玉米副产品饲料中,瘤胃合成微生物蛋白(MCP)和小肠真可吸收微生物蛋白(AMCP)含量与蛋白质酰胺Ⅰ带与非结构性碳水化合物之间的峰面积比值(AmideⅠ_TNSCHO)无显著相关性(P>0.05),但与其他各光谱分子功能团特征参数存在显著负相关(P<0.05)。AmideⅠ_TCHO和AmideⅡ_SCHO可共同作为预测因子估测小肠真可吸收瘤胃非降解蛋白(ARUP)、代谢蛋白(MP)和FMV,其决定系数均为0.997。本试验结果表明,不同玉米副产品基于NRC-2001模型下代谢蛋白预测产量与分子功能团特征参数之间存在相关关系,可利用傅立叶变换红外光谱分析技术对饲料营养价值进行快速分析和估测。
The experiment was aimed to predict the metabolic protein production of different corn by-products based on the NRC-2001 model,and analyze the molecular functional group characteristics of different corn by-products and the correlation between them based on the spectral technique,and establish a regression equation.The nutrient value of dairy cow protein was evaluated by NRC-2001 model,and the spectral data was collected and analyzed by Fourier transform infrared(FTIR)technique.The results showed that the metabolic parameters of different corn by-products with significant differences(P<0.05)predicted by the NRC-2001 model.Among them,the total amount of truly absorbable metabolic protein in the small intestine had the highest value in corn gluten meal,followed by corn germ cake,corn germ,corn gluten feed and corn kernel.There was a similar trend in feed milk value(FMV).In the spectral structure analysis,there were significant differences in the molecular functional group parameters of the feed(P<0.05).There was a correlation between the predicted yield of protein metabolites and the molecular functional group parameters.The regression model established was better.Among them,the value of AmideⅠ_CELC in corn kernel was significantly higher than other feeds(P<0.05),the values of AmideⅠ_SCHO and AmideⅠ_CELC were significantly lower in corn germ(P<0.05),the values of AmideⅡ_SCHO and AmideⅡ_CELC were significantly lower in corn gluten feed than that others(P<0.05),and the value of AmideⅡ_TNSCHO of the corn germ cake was significantly higher than that of other feeds(P<0.05).There was no significant correlation between the contents of MCP,AMCP and AmideⅠ_TNSCHO in corn and its by-products(P>0.05).However,there was a significant negative correlation between the characteristic parameters and other spectral molecular functional groups(P<0.05).AmideⅠ_TCHO and AmideⅡ_SCHO could be used as predictors to estimate ARUP,MP and FMV,with a coefficient of determination of 0.997.In conclusion,there was a relationship between the predicted metabolic parameters of protein and biomolecular spectroscopic features in different corn by-products and the nutritional value of feed could be quickly analyzed and estimated by the Fourier transform infrared spectroscopy.
作者
孙凯晶
赵洪波
杨金山
刘岩
刘帅
辛杭书
SUN Kaijing;ZHAO Hongbo;YANG Jinshan;LIU Yan;LIU Shuai;XIN Hangshu(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;Experimental Internship and Demonstration Center,Northeast Agricultural University,Harbin 150030,China)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第11期3033-3042,共10页
China Animal Husbandry & Veterinary Medicine
基金
黑龙江省科学基金项目(留学归国人员科学基金LC2017011)
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016132)