胶原诱导与抗原诱导C57BL/6小鼠类风湿关节炎造模的比较研究

Comparison of collagen-induced and antigen-induced C57BL/6 mouse models of rheumatoid arthritis

目的通过对C57BL/6小鼠背景下胶原诱导性关节炎(collagen-induced arthritis,CIA)和抗原诱导的关节炎(antigen-induced arthritis,AIA)造模情况的分析,来比较其造模成功率和关节炎进展的差异性。方法将42只小鼠分成两组,一组为CIA,一组为AIA。每组3只作为正常组,18只作为模型组。最后一次造模后定期监测小鼠的体重、膝关节和踝关节直径。结果在C57BL/6背景下,CIA造模成功率不高且严重程度差异大,AIA造模成功率高但维持时间短。结论为了更全面地研究类风湿性关节炎(rheumatoid arthritis,RA)的致病机制,可能更适合在后续的转基因小鼠身上造CIA模型。

Objective To analyze and compare the success rate of establishment and arthritis progression of collagen-induced(CIA)and antigen-induced(AIA)C57BL/6 mouse models of rheumatoid arthritis.Methods Forty-two C57BL/6 mice were divided into CIA and AIA groups.Three rats served as the normal group and 18 rats were used in each model group.After the last modeling,body weight and diameters of the knee joint and ankle of mice were monitored regularly.Results Under the C57BL/6 background,the success rate of establishment of CIA model was 41%,and the arthritis was maintained longer but less severe than that of AIA-induced models.The success rate of AIA modeling was 100%,and the arthritis was more severe but maintained for a shorter time.Conclusions To study RA pathogenesis more comprehensively,it may be more suitable to establish a CIA model in transgenic mice.