水稻种子不同活力测定方法的比较

Comparative Research on the Testing Methods of Different Seed Vigor in Rice

【目的】为筛选出一种或多种与田间出苗试验密切相关的室内水稻种子活力测定方法。【方法】选取不同类型的8个水稻品种为材料,开展标准发芽、幼苗生长、胚根突出、加速老化、冷处理发芽以及田间出苗试验。【结果】冷处理试验、25℃胚根突出试验第96小时和30℃第66小时的发芽率均与田间出苗率呈极显著正相关,相关系数分别为0.942和0.876;而幼苗生长试验25℃第10天的幼苗芽长(r=-0.848)和30℃第7天的幼苗芽长(r=-0.902)与田间出苗率呈极显著负相关关系。同时冷处理试验与胚根突出25℃、96 h之间呈极显著相关(r=0.913)、与胚根突出试验30℃、66 h (r=0.797)以及30℃第7天的幼苗芽长(r=-0.761)为显著相关。此外,30℃第7天的幼苗芽长与胚根突出试验25℃、96 h的计数(r=-0.750)显著相关;胚根突出试验中的25℃第96小时计数和30℃第66小时计数为极显著相关(r=0.872)。【结论】冷处理发芽试验、胚根突出试验(25℃第96小时和30℃第66小时计数)可推荐作为水稻种子活力测定方法。

[Purpose]The aim of this study was to find one or more inexpensive,uncomplicated,rapid,objective,reproducible,and related to field emergence for rice seeds vigor testing methods.[Method]In this study,8 different type rice varieties as experiment materials,the following tests were used which containing:standard germination test,seedling growth test(the root length and shoot length of 7,10,14 days were measured at 25℃and 30℃,respectively),radicle emergence test(25℃and 30℃),accelerated aging test,cold treatment test and field emergence test.[Results]The results of correlation analysis between tests showed that the first cold treatment test,radicle emergence test of 25℃at 96 h(r=0.942)and 30℃at 66 h(r=0.876),and 25℃seedling growth test on the 10 d of shoot length(r=?0.848)and on the 7 d of the shoot length(r=?0.902)were extremely significantly correlated with field emergence.Secondly,the cold treatment test and radicle emergence test of 25℃at 96 h(r=0.913)was extremely significant correlation,and radicle emergence test of 30℃at 66 h(r=0.876)was significantly correlated.The radicle emergence test between 25℃by 96 h count and 30℃by 66 h count was extremely significant correlation(r=0.872).[Conclusion]In view of the above results,cold treatment test,the radicle emergence test(25℃by 96 h and 30℃by 66 h count)should be recommendation for a vigor testing methods in rice seeds.