NQO1和HO-1在T细胞淋巴瘤中的表达及意义

Expression and significance of NAD (P) H:quinone oxidoreductase 1 and heme oxygenase-1 proteins in T-cell lymphoma

目的:探讨醌氧化还原酶1[NAD(P)H:quinine oxidoreductase 1,NQO1]与血红素加氧酶-1(heme oxygenase-1,HO-1)在T细胞淋巴瘤(T-cell lymphoma,TCL)中的表达及其临床病理特征间的关系。方法:回顾性分析2012年12月至2018年5月甘肃省人民医院确诊的61例TCL患者的临床资料。采用免疫组织化学法检测NQO1和HO-1在61例TCL组织(TCL组)及20例淋巴结反应性增生组织(对照组)中的表达水平。结果:NQO1和HO-1在TCL组织中的阳性表达率均明显高于淋巴结反应性增生组织,差异具有统计学意义(P<0.05);NQO1与TCL临床分期和B症状有关(P<0.05);HO-1与TCL的临床分期、骨髓侵犯和B症状密切相关(P<0.05);二者与TCL患者的年龄、性别、乳酸脱氢酶水平及病理分型无关(P>0.05);NQO1和HO-1的表达存在相关性(r=0.264;P=0.040)。结论:NQO1和HO-1在TCL中高表达,可能互相作用参与TCL的发生发展。

Objective:To investigate the expression of NAD(P)H:quinone oxidoreductase 1(NQO1)and heme oxygenase-1(HO-1)in Tcell lymphoma(TCL),and investigate the correlation between these two indicators and other clinicopathological parameters in TCL.Methods:Clinical data of 61 patients with TCL who were initially diagnosed in Gansu Provincial Hospital were analyzed retrospectively.Immunohistochemical examination was performed to detect NQO1 and HO-1 expression levels in 61 TCL tissues(TCL group)and 20 lymph node reactive hyperplasia tissues(control group).Results:Positive expression rates of NQO1 and HO-1 were significantly higher in TCL tissues than in lymph node reactive hyperplasia tissues(P<0.05).NQO1 expression was closely related with Ann-Arbor clinical stage and B symptoms(P<0.05);HO-1 expression was correlated with clinical stage,bone marrow invasion,and B symptoms(P<0.05).NQO1 and HO-1 expression levels were not related to age,sex,lactate dehydrogenase level,and pathological type(P>0.05);there was a correlation between NQO1 and HO-1 expression(r=0.264;P=0.040).Conclusions:NQO1 and HO-1 are highly expressed in TCL and may interact and contribute to the occurrence and development of TCL.