钛铜合金纳米管形貌能够降低细菌活性并促进成骨细胞功能（英文）

Titanium-copper alloys with nanotubular coatings increase antibacterial abilities and osteoblast functions

背景:植入物的感染仍然是严重的骨科术后不良反应。铜是一种目前已知具有抗菌性能的金属。研究表明,通过纳米技术制备纳米结构金属可以促进成骨细胞在体内的黏附、增殖和骨结合。临床上最常见的重要植入物相关病原菌-金黄色葡萄球菌,是用来测试抗菌性能的钛-铜合金纳米管。目的:观察钛-铜合金纳米管的抗菌能力及对成骨细胞功能的影响。方法:实验将不同材料(纯钛、钛纳米管及5%-钛铜合金纳米管)与小鼠成骨细胞(MC-3T3-E1)共培养6,24h,观察细胞在支架上的黏附和增殖。比较了钛-铜合金纳米管、纯钛纳米管和纯钛的抗菌性能。在各组材料形貌表面种植成骨细胞检测其生物相容性,在各组材料表面种植金黄色葡萄球菌检测其抗菌性能。结果与结论:(1)扫描电镜可见,钛纳米管和钛-铜合金纳米管表面良好的细胞黏附性,小鼠成骨细胞形态良好,排列规则;纳米管组细胞增殖情况优于纯钛组,而钛-铜合金纳米管与钛纳米管组差异无显著性意义;(2)细菌黏附实验显示,与钛铜合金纳米管相比,钛金属和TiO2纳米管上的细菌数量更多;(3)结果证实,钛-铜合金纳米管对细菌黏附具有良好的抑制作用,不影响成骨细胞的生物功能。

BACKGROUND:Implant infection is still a serious adverse event after orthopedic surgery.Copper(Cu)is a currently known metal that has antibacterial properties.Studies have shown that nanostructured metals prepared by nanotechnology can promote the adhesion,proliferation and osseointegration of osteoblasts in vivo.Staphylococcus aureus,the most common implant-related pathogen in clinical practice,is used to test antibacterial properties of titanium-copper alloy nanotubes.OBJECTIVE:To observe the effect of antibacterial properties of titanium-copper alloy nanotubes on the function of osteoblasts.METHODS:Mouse osteoblasts(MC-3T3-E1)were co-cultured with different materials,including pure titanium,titanium dioxide nanotubes,and titanium-copper alloy nanotubes with a copper content of 5%,for 6 and 24 hours.Cell adhesion and proliferation on the scaffold were observed.Antibacterial properties of titanium-copper alloy nanotubes,titanium dioxide nanotubes and pure titanium were compared.Biocompatibility of osteoblasts co-cultured on different material surfaces was detected,and antibacterial properties of different materials to Staphylococcus aureus were measured.RESULTS AND CONCLUSION:(1)Under scanning electron microscope,we observed good cell adhesion onto the surface of titanium dioxide nanotubes and titanium-copper alloy nanotubes,and the adherent cells had good cell morphology and regular arrangement.Cell proliferation of osteoblasts was better in the two nanotube groups than in the pure titanium group,but there was no significant difference between the titanium-copper alloy nanotube and titanium dioxide nanotube groups.(2)Higher bacteria counts were observed in the pure titanium and titanium dioxide nanotube groups than the titanium-copper alloy nanotube group.In conclusion,the titanium-copper alloy nanotubes have good inhibitory effect on bacterial adhesion,and have no influence on the bio-functions of osteoblasts.