脂肪间充质干细胞条件培养液联合富血小板纤维蛋白修复小鼠皮肤损伤

Adipose tissue-derived mesenchymal stem cell conditioned medium combined with platelet-rich fibrin repairs skin injury in mice

背景:富血小板纤维蛋白可促进软组织愈合,间充质干细胞分泌的可溶性因子具有促进皮肤再生的作用,两者联合应用促进皮肤损伤修复尚未见报道。目的:探讨脂肪间充质干细胞条件培养液联合富血小板纤维蛋白对小鼠皮肤创伤愈合的影响。方法:制作C57/BL6小鼠(西南医科大学实验动物中心提供)全层皮肤损伤模型,随机分为4组,每组10只。自造模后即开始治疗,各组分别涂抹脂肪间充质干细胞专用培养基、脂肪间充质干细胞条件培养液、富血小板纤维蛋白、脂肪间充质干细胞条件培养液+富血小板纤维蛋白,3次/d,连续7d。在治疗后的1,3,7,10 d测量创面大小并计算治疗后3,7 d的创面愈合率;治疗后第14天进行创面愈合的组织学分析。结果与结论:(1)与脂肪间充质干细胞专用培养基组相比,其他3组创面愈合时间缩短;(2)治疗后3,7 d,脂肪间充质干细胞条件培养液+富血小板纤维蛋白组的创面愈合率高于其他3组,脂肪间充质干细胞条件培养液组和富血小板纤维蛋白组的创面愈合率高于脂肪间充质干细胞专用培养基组,差异有显著性意义(P <0.05);(3)组织学分析显示,脂肪间充质干细胞条件培养液+富血小板纤维蛋白组小鼠创面白细胞及成纤维细胞聚集、血管新生、肉芽组织重塑、上皮化均比其他3组明显,伤口愈合效果最好;(4)结果表明,脂肪间充质干细胞条件培养液与富血小板纤维蛋白联合应用能有效促进小鼠皮肤损伤的修复,效果优于单独应用脂肪间充质干细胞条件培养液或富血小板纤维蛋白。

BACKGROUND:Platelet-rich fibrin can promote soft tissue healing,and soluble factors secreted by mesenchymal stem cells can promote skin regeneration,but their combination effects on skin repair are little reported.OBJECTIVE:To investigate the effect of conditioned medium of adipose tissue-derived mesenchymal stem cells combined with platelet-rich fibrin on the healing of skin wound in mice.METHODS:Full-thickness skin injury models were established in C57/BL6 mice and randomly divided into four groups,10 mice in each group.After the modeling,each group was smeared with the specific medium of adipose tissue-derived mesenchymal stem cells,the conditioned medium of adipose tissue-derived mesenchymal stem cells,the platelet-rich fibrin,the conditioned medium of adipose tissue-derived mesenchymal stem cells combined with platelet-rich fibrin,thrice daily for 7 consecutive days.The wound size was measured at 1,3,7 and 10 days after treatment and the wound healing rate was calculated at 3 and 7 days after treatment.Histological analysis of wound healing was performed at 14 days after treatment.RESULTS AND CONCLUSION:Compared with the specific culture medium of adipose tissue-derived mesenchymal stem cells,the wound healing time in the other three groups was shorter.The wound healing rate of adipose-derived mesenchymal stem cell conditioned medium combined with platelet-rich fibrin group was significantly higher than that in the other three groups at 3 and 7 days after treatment(P<0.05).The adipose-derived mesenchymal stem cell conditioned medium group and platelet-rich fibrin group showed higher healing rate than the adipose-derived mesenchymal stem cell specific medium group(P<0.05).Histological findings showed that the aggregation of white blood cells and fibroblasts,angiogenesis,granulation tissue remodeling and epithelization in the conditioned medium of adipose mesenchymal stem cells combined with the platelet-rich fibrin group were more obvious than those in the other three groups,in which the best wound healing effect was found.These results suggest that the combination of adipose tissue-derived mesenchymal stem cell conditioned medium and platelet-rich fibrin can effectively promote the repair of skin injury in mice,and the effect is better than that of conditioned medium or platelet-rich fibrin alone.