陕西关中地区苹果褐斑病菌遗传多样性分析

Genetic Diversity of Diplocarpon mali Isolated from Guanzhong Plain of Shaanxi Province

为了揭示苹果褐斑病菌(Diplocarpon mali)的群体遗传多样性,利用ISSR分子标记技术,通过ISSR-PCR扩增,对分离自陕西省关中平原7个县(区)的64个菌株进行遗传多样性分析。结果显示,陕西关中地区苹果褐斑病菌的遗传多样性丰富,不同县(区)病菌的遗传多样性存在差异,渭南市白水县病菌的遗传多样性水平相对较高,咸阳市乾县相对较低。群体之间的遗传分化系数不同,遗传变异主要发生在群体内;不同地区群体之间存在不同程度的基因交流,其中关中中部群体的基因交流更广泛;关中东部、中部、西部地区之间的群体也存在基因交流,其中东部和西部之间群体的基因交流更频繁。NTsys聚类结果表明,种群之间的遗传亲缘关系与分离株的地理来源没有明显的相关性。

In order to detect the population genetic diversity of apple brown spot pathogens(Diplocarpon mali),the inter simple sequence repeat(ISSR)marker was used to analyze 64 isolates collected from 7 locations at Guanzhong plain of Shaanxi province.The results indicated that D.mali populations had abundant genetic diversity,which was various in different regions.The genetic diversity level was relatively high in Baishui county but low in Qianxian county.The genetic differentiation coefficient of D.mali populations varied in different areas,and the differentiation was mainly observed within population.Gene flow was found among populations,and it was more frequent between eastern and western regions of Guanzhong plain.Population cluster analysis by NTsys showed there was no significant correlation between genetic relationship and their geographical origins.