便携式拉曼光谱仪快速检测唾液中盐酸吡格列酮的含量

Rapid and Quantitative Detection of Pioglitazone Hydrochloride in Human Saliva by Portable Raman Spectrometer

人体唾液与血液中的相应成分有着密切关系。利用唾液代替血液进行检测,可极大地缩短分析时间、减少检测限制、降低安全隐患等,因此在临床医学、毒品管控等方面均有重要意义。发展了便携式拉曼光谱仪利用表面增强拉曼光谱技术快速定量检测唾液中盐酸吡格列酮(口服降血糖药物)含量的方法。借助纳米金溶胶的表面增强拉曼散射效应,在激发光源波长为785 nm时,可以得到低浓度盐酸吡格列酮的高质量拉曼光谱图。同时,不同浓度盐酸吡格列酮表面增强拉曼光谱分析结果表明,该方法还可直接用于唾液中盐酸吡格列酮的定量检测。盐酸吡格列酮含量与其特征峰强度线性相关,相关系数为0. 992 3,且最低检测浓度达10μg·L^(-1)。

The components of saliva are closely related to the corresponding components in blood.Using saliva instead of blood for detecti on can greatly reduce analysis time,limitation and potential safety hazard,thus it is of great importance to clinical research and control of drugs.In this w ork,combined with a portable Raman spectrometer,quantitative detection of piog litazone hydrochloride(an oral hypoglycemic drug)in saliva has been realized by surface-enhanced Raman spectroscopy(SERS).With this method,the characteris tic Raman peaks for pioglitazone hydrochloride can be easily observed.Furthermo re,the SERS method can be used to quantitatively detect the pioglitazone hydroc hloride in saliva.The concentration of pioglitazone hydrochloride is linearly c orrelated to its Raman intensity with a coefficient of determination(R2)of 0.992 3,and the minimum detectable concentration can be as low as 10μg·L^-1.