甲状腺乳头状癌组织中CELF1表达及其功能研究

CELF1 gene expression and its function in papillary thyroid carcinoma tissues

目的:探讨CELF1在甲状腺乳头状癌(PTC)组织中表达及对PTC细胞增殖和侵袭力的影响。方法:免疫组化法检测PTC组织和癌旁组织中CELF1蛋白表达,小分子RNA构建CELF1低表达细胞系,实时荧光定量PCR技术检测CELF1表达,CCK-8法和克隆形成实验检测细胞增殖能力,Transwell小室检测细胞侵袭能力。结果:PTC组织中CELF1蛋白阳性表达率较高,且与TNM分期、侵犯被膜和淋巴结转移有关(P<0.05);与对照序列组和空白组相比,CELF1干扰序列组细胞中CELF1 mRNA相对表达量降低(P<0.05),细胞增殖水平降低(P<0.05),侵袭细胞数降低(P<0.05)。结论:CELF1蛋白在PTC组织中呈高表达,特异性下调PTC细胞中CELF1基因表达可减少细胞增殖,抑制细胞侵袭力。

Objective:To investigate the expression of CELF1 protein in the tissues of papillary thyroid carcinoma(PTC)and the effects of CELF1 expression on PTC cell proliferation and invasiveness.Methods:Immunohistochemistry was used to detect the expressions of CELF1 proteins in PTC tissues and adjacent tissues.CELF1 low expression cell line was constructed by small interfering RNA.CELF1 expression was determined using real-time fluorescence quantitative PCR,and cell proliferation was detected using CCK-8 and clonogenic assay.Transwell chambers was used to detect cell invasiveness.Results:The positive expression rate of CELF1 protein was higher in PTC tissues,and correlated with TNM stage,invasion of envelope and lymph node metastasis(P<0.05).Compared with the control sequence group and the blank group,decreased relative expression level of CELF1 mRNA,cell proliferation and the number of invasive cells were seen in CELF1 interference sequence group(all P<0.05).Conclusion:CELF1 protein is highly expressed in PTC tissues.Specific down-regulation of CELF1 gene expression in PTC cells could reduce cell proliferation and inhibit cell invasiveness.