1种野生多孔菌的分离鉴定及其高产漆酶培养基的筛选

Isolation and Classification of a Wild Polypore and Screening the Medium for High-yield of Laccase

对内蒙古自治区锡林郭勒盟境内的1种野生多孔菌进行分子鉴定。通过组织分离的方法对该菌种进行分离培养,并选取常用的4种固体培养基进行最佳固体培养基配方的筛选;以漆酶活性为评价指标,筛选高产漆酶的液体培养基。结果表明,该种野生多孔菌为硬毛粗盖孔菌(Coriolopsis trogii)。固体培养基D(葡萄糖20 g、蛋白胨5 g、酵母浸膏10 g、KH2PO42 g、MgSO41 g、琼脂20 g,加水至1 000 mL,自然pH值)为该种多孔菌的最佳固体培养配方,在该培养基上培养的该种野生多孔菌菌丝长势良好,菌丝干质量为0.132 g; 2号液体培养基(马铃薯切片200 g、葡萄糖20 g、蛋白胨2 g、酵母浸膏2 g、KH2PO43 g、MgSO41.5 g,加水至1 000 mL,自然pH值)为该种多孔菌发酵产漆酶的最佳液体培养配方,在该培养基上培养的该种野生多孔菌菌丝生物量为8.56 g/L,漆酶活性可达907.34 U/L。

In this paper,a wild polypore collected from Xilin Gol in Inner Mongolia was classified using molecular method and isolated for the culture of mycelia by tissue isolating.In addition,the optimal solid medium was assayed from the reported solid media.The laccase activity was used as an evaluation index to screen the liquid media producing high activity laccase.The results showed that the wild polypore was Coriolopsis trogii,the optimal solid medium was D(Glucose 20 g,peptone 5 g,yeast extract 10 g,KH 2PO 4 2 g,MgSO 4 1 g,agar 20 g,adding water to 1 000 mL,natural pH),in which the mycelia growth well and the mycelium dry weight was 0.132 g.The optimal liquid medium was No.2(Potato slices 200 g,glucose 20 g,peptone 2 g,yeast extract 2 g,KH 2PO 4 3 g,MgSO 4 1.5 g,adding water to 1 000 mL,natural pH)in which the mycelial biomass and laccase activity were 8.56 g/L and 907.34 U/L,respectively.