副猪嗜血杆菌河南流行株的分离鉴定及分型

Isolation,Identification and Typing of Haemophilus parasuis Strains in Henan Province

为了解河南省副猪嗜血杆菌病的流行情况,对2017—2018年采自河南省不同地区的45份疑似病料,进行细菌分离及形态学观察、培养特征鉴定、PCR鉴定及分型。结果显示,共分离鉴定出12株副猪嗜血杆菌,血清型为1、2、4、5、7、13型和不可分型。其中,血清型1型2株,分别命名为XX-3、HB-1;血清型2型1株,命名为XX-4;血清型4型1株,命名为ZJ-1;血清型5型4株,分别命名为LY-1、DF-1、XX-2、KF-1;血清型7型2株,分别命名为SQ-2、XX-1;血清型13型1株,命名为NY-1;还有1株不可分型,命名为SQ-1。可见,河南省存在多个副猪嗜血杆菌血清型,且不同地区血清型不一致,1、5、7型为优势血清型。

In order to find out the prevalence of Haemophilus parasuis in Henan Province,45 samples from different areas of Henan Province from 2017-2018 were observed on bacterial morphology,identified by culture characteristics and detected by 16S rRNA gene PCR.Results a total of 12 Haemophilus parasuis strains were isolated and identified.The isolates included 2 strains of serotype 1,were named as XX-3,HB-1,1 strains of serotype 2,was named as XX-4,4 strains of serotype5,were named as LY-1,DF-1,XX-2,KF-1,2 strains of serotype 7,were named as XX-3,HB-1,1 strains of serotype 13,was named as NY-1,and 1 unserotypeable strains in sick pigs.The results showed that there were many serotypes of Haemophilus parasuis in Henan Province,and the serotypes were different in different regions.Serotypes 1,5 and 7 were the dominant serotypes.