红曲色素萃取发酵中表面活性剂的分离与重复利用

Separation and Reutilization of Surfactant in Extractive Fermentation of Monascus Pigment

研究三氯甲烷萃取-大孔树脂吸附(trihalomethanes extraction combined with resin absorption,THMS-Resin)对萃取发酵液中红曲色素的分离能力和Triton X-100回收重复利用的萃取性能。结果显示,THMS-Resin法能够很好地分离非离子表面活性剂Triton X-100和红曲色素,Triton X-100回收率达到78%;红曲黄色素回收率达到52%左右,同时具有显著的抗氧化活性。将回收的Triton X-100重复用于红曲霉萃取发酵,细胞生长和色素代谢良好,并且萃取剂保持较好的萃取分离性能。采用合适比例的新旧Triton X-100混合进行红曲霉萃取发酵,能显著促进红曲霉胞内外色素代谢,总色素产量最大提高35%左右。Triton X-100经过5次重复萃取发酵,每次回收率均可达到80%左右;并且各批次红曲霉细胞生长稳定,萃取效率和代谢能力强,胞外黄色素相对含量达到1.0,胞内黄色素相对含量维持0.6以上。该分离方法能够获得较高含量的黄色素,促进Triton X-100的重复利用,具有很好的应用潜力。

In this paper,pigment separation by trihalomethanes extraction combined with resin absorption(THMSResin)was investigated.The performance of the recovered Triton X-100 in the extractive fermentation was also studied.Results showed that the Monascus pigments and Triton X-100 could be well separated through the combined method.The recovery of Triton X-100 and yellow pigments with high antioxidant activity was 78%and 52%,respectively.The recycled Triton X-100 exhibited excellent performance for cell growth and pigment metabolism while maintaining the extraction performance.Meanwhile,the extractive fermentation with a proper mixture of the new and recycled Triton X-100 could significantly promote the synthesis of both intracellular and extracellular Monascus pigment,resulting in a 35%increase of the total pigment yield.Five cycles of extractive fermentation were conducted.In each cycle,the average recovery of Triton X-100 was about 80%with a good biocompatibility;cell growth was stable and the extraction efficiency and metabolism were high.The relative content of extracellular and intracellular yellow pigment was 1.0 and 0.6,respectively.This method showed potential applications in obtaining high yield of Monascus yellow pigments while promoting the reuse of Triton X-100.