ClO_2杀菌复合壳寡糖涂膜对鲜食糯玉米的保鲜效果

Effect of ClO_2 Sterilization Combined with Chitosan Oligosaccharide Coating on Maintaining the Quality of Waxy Corn

分别采用ClO_2杀菌处理、壳寡糖(chitosan oligosaccharide,COS)涂膜处理以及ClO_2杀菌复合COS涂膜(ClO_2-COS)处理鲜食糯玉米,对比不同保鲜处理对鲜食糯玉米贮藏期间的抑菌情况、多酚含量、木质素含量、木质素积累速率、苯丙氨酸解氨酶活力、过氧化物酶活力的影响。结果发现:200 mg/L的ClO_2杀菌复合15 mg/mL COS涂膜处理表现出良好的抑菌能力,贮藏35 d时霉菌和酵母菌总数为(2.30±0.12)(lg(CFU/g)),显著低于ClO_2杀菌处理组((3.78±0.16)(lg(CFU/g)))和COS涂膜处理组((4.18±0.09)(lg(CFU/g)))(P<0.05);ClO_2-COS处理能够降低木质素积累速率,降低多酚含量并使其基本保持较低水平;ClO_2-COS处理可以抑制苯丙氨酸解氨酶活力过快上升,降低苯丙烷代谢水平,还能降低过氧化物酶活力,使其在贮藏期间一直保持较低水平,从而减少木质素积累。

In this paper,the separate and combined effects of chlorine dioxide(ClO2)sterilization and chitosan oligosaccharide(COS)coating on bacterial growth inhibition,polyphenol and lignin content,lignin accumulation rate,phenylalanine ammonialyase(PAL)activity and peroxidase(POD)activity in fresh waxy corn during storage were compared.It turned out that the combination of 200 mg/L ClO2 and 15 mg/mL COS exhibited a good inhibitory effect on pathogen infection.After 35 d of storage,total mold and yeast count in the combined treatment group was(2.30±0.12)(lg(CFU/g)),which was significantly lower than that in the ClO2((3.78±0.16)(lg(CFU/g)))and COS((4.18±0.09)(lg(CFU/g)))treatment groups(P<0.05).The combined treatment could reduce lignin accumulation rate,and reduce and maintain polyphenol content to a low level.Furthermore,the combined treatment inhibited the rapid increase of PAL activity and reduce the level of phenylpropane metabolism.It could also reduce POD activity and keep it at a low level during storage,thus reducing lignin accumulation.