循环肿瘤细胞富集技术研究进展

Progress in Enrichment Techniques of Circulating Tumor Cells

循环肿瘤细胞(CTCs)是从原发性或转移性肿瘤部位脱落至循环系统的极少量的肿瘤细胞,开展CTCs的系统研究对于肿瘤的早期诊断和预后评估具有重要的临床意义,在癌症个性化治疗、药物开发等方面具有潜在价值。但由于CTCs在血液中含量极少,富集技术成为制约其临床应用的瓶颈。此外,CTCs亚型分布及活性CTCs的分子鉴定和功能分析更有助于临床诊断和个性化治疗,因此对CTCs的富集技术提出了更高的要求。本文综述了近年来CTCs分离富集、亚型捕获及捕获后无损释放方面的研究进展,并探讨了其面临的挑战和发展趋势。

Circulating tumor cells(CTCs)are rare tumor cells shed from primary and metastatic tumor sites into the circulation and have great clinical significance for the early diagnosis and prognosis of cancer patients,as well as have potential value in personalized cancer therapy and drug development.However,CTCs are often rare in blood sample.Therefore,the development of enrichment techniques is turning out to be the bottleneck of its clinical application.Additionally,the distribution of CTCs subtypes and the molecular identification and functional analysis of viable CTCs are more helpful for clinical diagnosis and personalized cancer therapy,and higher requirements for CTCs enrichment technique are put forward.In this paper,the progress in enrichment of CTCs and its subtype capture and post-capture nondestructive release in recent years were reviewed,and the future trends of CTCs enrichment technologies including the challenge and opportunity were discussed.