以杆菌肽为模板的金簇制备及其在腺嘌呤核苷三磷酸检测中的应用

Fabrication of Bacitracin-Templated Gold Nanocluster and Its Application in Detection of Adenosine Triphosphate

建立了一种以杆菌肽(Bacitracin)为还原剂和模板制备金纳米簇的方法,并利用腺嘌呤核苷三磷酸(ATP)能恢复Fe^(3+)淬灭的金纳米簇荧光的特性,设计了一种"点亮"型金纳米簇荧光探针,用于ATP的检测。在pH=1的水溶液中,Bacitracin与HAuCl_4的摩尔比为1∶1时,室温下反应2 h,成功制备了有较强荧光的金纳米簇AuNCs@Bacitracin。淬灭剂Fe^(3+)浓度为5 mmol/L时,对AuNCs@Bacitracin的荧光淬灭程度达98%。基于AuNCs@Bacitracin-Fe^(3+)复合物建立了一种检测ATP的方法,ATP浓度为10μmol/L～8 mmol/L范围内时,lg(F/F0)与浓度呈线性关系,R^2=0.9902。利用此方法对加标人工血清样品进行检测,3个加标水平下ATP的回收率为103.0%～105.0%,RSD<1.0%,结果准确可靠。

A novel method for fabrication of gold nanocluster by using bacitracin as a reducing agent and stabilizer in aqueous solution was developed.The fluorescence of bacitracin-templated gold nanoclusters(AuNCs@Bacitracin)could be quenched in the presence of Fe 3+.The property of fluorescence quenched by Fe 3+could be applied to preparation of a“turn-on”probe for detection of adenosine triphosphate(ATP).Under the optimized conditions,AuNCs were prepared by the reaction of bacitracin and HAuCl 4 with the molar ratio of 1∶1 at pH=1 for 2 h.98%of original intensity of AuNC fluorescence was quenched in the presence of 5 mmol/L Fe 3+.Besides,the value of lg(F/F 0)was linearly related with the concentration of ATP(R 2=0.9902)in the range of 10μmol/L-8 mmol/L.The fabricated probe was then applied to detection of the level of ATP in artificial serum with recovery of 103.0%-105.0%and RSD<1.0%,which suggested that the method was accurate and reliable.