期刊文献+

发酵豆酱中ACE抑制肽的制备及分离纯化 被引量:2

Preparation,Isolation and Purification of Angiotensin-Converting Enzyme Inhibitory Peptide from Fermented Soybean Paste
下载PDF
导出
摘要 为了获得ACE抑制肽,利用微生物发酵法以大豆为原料制成中国传统豆酱,通过研磨、溶解、超滤后获得了ACE抑制活性最高的肽段区间3~10k,并依次利用DEAE琼脂糖微球a、Sephadex G-75凝胶对该组分进行分离纯化,最后纯化获得组分DGIII的ACE抑制活性最高,达到(98.3±1.24)%,IC50为0.08mg/mL,说明豆酱具有开发高抑制率ACE食品的潜力。 In order to achieve ACE inhibitory peptide,Chinese traditional soybean paste is made from soybean by microbial fermentation.After grinding,dissolving and ultrafiltration,the peptide segment with the highest ACE inhibitory activity is obtained in the range of 3~10 k.And then it is purified by DEAE agarose ion exchange column and Sephadex G-75 gel column.Finally,the purified component DGIII with the highest ACE inhibitory activity is obtained,with the inhibitory activity of(98.3±1.24)%,and the IC 50 is 0.08 mg/mL.It shows that soybean paste development has a high potential to inhibit ACE foods.
作者 李利军 马英辉 卢美欢 LI Li-jun;MA Ying-hui;LU Mei-huan(Shaanxi Institute of Microbiology,Xi'an 710043,China)
出处 《中国调味品》 CAS 北大核心 2018年第12期71-75,共5页 China Condiment
基金 陕西省科技攻关重点项目(2015SF165)
关键词 豆酱 ACE抑制肽 分离纯化 soybean paste ACE inhibitory peptide isolation and purification
  • 相关文献

参考文献7

二级参考文献41

  • 1周爱国,黄玉波,王开贺,徐本峰,宋思琴,魏红山.检测血管紧张素转化酶在AFP阴性肝癌诊断中的价值[J].实用肝脏病杂志,2003,6(1):19-20. 被引量:1
  • 2邵伟,熊泽,何晓文.发酵大豆多肽及其功能研究[J].食品科技,2005,30(4):26-28. 被引量:39
  • 3孙天松,孟和毕力格,王俊国,陈永福,赵电波,张和平.新疆维吾尔自治区酸马奶化学组成分与微生物学分析[J].中国乳品工业,2005,33(10):9-13. 被引量:25
  • 4CUSHMAN D W, CHEUNG H S, SABO E F, et al. Development and Design of Specific Inhibitors of Angiotensin-Converting Enzyme [J]. The American Journal of Cardiology, 1982, 49(6):1390- 1394.
  • 5RREIRA I M P L V O, ECA R, PINHO O, et al. Development and Validation of an HPLC/UV Method for Quantification of Bioactive Peptides in Fermented Milks [J]. Journal of Liquid Chromatography and Related Technologies, 2007, 30(13-16):2139 -2147.
  • 6FERREIRA I M P L V O, PINFIO O, MOTA M V, et al. Preparation of Ingredients Containing an ACE-Inhibitory Peptide by Tryptic Hydrolysis of Whey Protein Concentrates [J]. International Dairy Journal, 2007, 17(5):481 - 487.
  • 7FUJITA H, YOKOYAMA K, YOSHIKAWA M. Classification and Antihypertensive Activity of Angiotensin Ⅰ -Converting Enzyme Inhibitory Pepddes Derived from Food Proteins [J]. Journal of Food Science, 2000, 65(4):564 - 569.
  • 8CURTIS J M, DENNIS D, WADDELL D S, et al. Determination of Angiotemin-Converting Enzyme Inhibitory Peptide Leu - Lys - Pro - Asn - Met (LKPNM) in Bonito Muscle Hydrolysates by LC - MS/MS [J]. Journal of Agricultural and Food Chemistry, 2002, 50 (14):3919 - 3925.
  • 9CUSHMAN D W, CHEUNG H S. Spectrophotometric Assay and Properties of the Angiotensin-Converting Enzyme of Rabbit Lung [J]. Biochemical Pharmacology. 1971, 20(7): 1637-1648.
  • 10WU J, DING X. Characterization of Inhibition and Stability of Soy-Protein-Derived Angiotensin - Ⅰ -Converting Enzyme Inhibitory Pepfides [J]. Food Research International, 2002, 35 (4): 367- 375.

共引文献78

同被引文献20

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部