响应面法优化羊肚菌可溶性膳食纤维提取工艺

Optimization of Soluble Dietary Fiber from Morchella esculenta by Response Surface Methodology

以北川干制羊肚菌为原料,通过单因素试验和响应面试验对化学法提取羊肚菌中可溶性膳食纤维的工艺条件进行优化,研究料液比、碱液浓度、提取温度和提取时间对羊肚菌可溶性膳食纤维得率的影响。结果表明,最佳工艺参数为料液比1∶20(g/mL)、提取液浓度0.75%、提取温度63℃、提取时间60min,在此工艺条件下羊肚菌可溶性膳食纤维得率为33.06%。

The extraction conditions of soluble dietary fiber from Morchella esculenta by alkali extraction based on single factor experiment and response surface methodology are studied.The effects of solid-liquid ratio,NaOH concentration,extraction temperature and extraction time on the yield of SDF are investigated.The results show that the optimal extraction conditions are as follows:solid-liquid ratio is 1∶20(g/mL),NaOH concentration is 0.75%,extraction temperature is 63℃and extraction time is 60 min,under these conditions,the maximum yield of SDF is 33.06%.