骨折模型小鼠周围组织E、P选择素含量变化及意义

Alteration and significance of E-selectin and P-selectin in the tissues surrounding fracture in mouse models

背景:E、P选择素是重要的黏附分子,参与机体内的炎症反应,而炎症反应对早期骨折的愈合具有积极的促进作用。目的:探究小鼠骨折周围组织内E、P选择素含量变化及意义。方法:取BALB/c小鼠36只,制作小鼠稳定胫骨骨折模型,建模后随机将小鼠分为1 h、6 h、12 h、24 h、48h及3d组,分别在对应时间点取骨折周围血肿肌肉组织,健侧同部位胫骨周围肌肉组织作为对照,采用酶联免疫吸附法对所取组织标本进行E、P选择素含量检测。结果与结论:①骨折周围组织的E、P选择素含量均较健侧胫骨周围肌肉组织明显升高(P<0.01),其中E选择素含量在6 h左右达到高峰,P选择素含量在24 h左右达到峰值;②小鼠胫骨骨折后,骨折周围组织内E、P选择素含量明显上调,提示两者在小鼠骨折早期内可能介导了炎症细胞迁移、黏附,并参与了骨折后机体修复的病理过程。

BACKGROUND:E-selectin and P-selectin are important adhesion molecules involved in the inflammatory response in vivo,and inflammatory response plays a positive role in promoting early fracture healing.OBJECTIVE:To investigate the alterations and significance of E-selectin and P-selectin in the tissues surrounding fracture of mice.METHODS:Thirty-six BALB/c mice were selected for establishing stable tibia fracture model.The mouse models were randomly divided into 1-,6-,12-,24-,and 48-hour and 3-day groups.The hematoma tissue surrounding the fracture was removed at corresponding time,and contralateral tibial tissues at the healthy side were used as controls.The concentrations of E-selectin and P-selectin in tissues were detected by ELISA.RESULTS AND CONCLUSION:The concentrations of E-selectin and P-selectin in the tissues surrounding the fracture were significantly higher than those in the healthy side(P<0.01).E-selectin reached a peak at about 6 hours.P-selectin was the highest at about 24 hours.In summary,the concentrations of E-selectin and P-selectin in the tissues surrounding the fracture were significantly up-regulated,suggesting that E-selectin and P-selectin may mediate the migration and adhesion of inflammatory cells during the early stage of mouse fracture and play an important role in the pathological process of bone repair.