摘要
基于八元瓜环(Q[8])可使吖啶橙(AD)的荧光降低的性质制备了荧光探针2AO@Q[8],当在该探针中加入多菌灵后荧光强度又逐渐增强,利用此超分子配合物的荧光效应,构建了一种能够检测多菌灵的超分子荧光探针.研究结果表明,该探针在水溶液中对多菌灵具有良好的选择性,在一定浓度范围内其线性关系良好,检出限为8. 14×10^(-8)mol/L.细胞成像结果显示,该探针在前列腺癌细胞中对多菌灵具有良好的响应,可用于生物细胞内多菌灵的识别检测.
A host-guest complex between cucurbit[8]uril(Q[8])and acridine orange(AO)was used as a fluorescent probe for detecting the carbendazim(CBZ).2AO@Q[8]fluorescent probe was designed and used to detect carbendazim with high sensitivity and selectivity with a detection limit of ca.10^-8 mol/L.A good linear relationship of emission intensity at ca.529 nm for CBZ at concentrations of 0.4-5.0μmol/L was observed.The proposed sensing mechanism was investigated via 1H NMR spectroscopy combined with density functional theory calculations at the B3LYP/6-31G(d)level.The cell imaging study showed that the 2AO@Q[8]complex could be used to detect carbendazim in prostate cancer(PC3)cells,which may help to sense carbendazim in biological system.
作者
杨梅
刘青
唐青
王成会
杨梅香
孙涛
黄英
陶朱
YANG Mei;LIU Qing;TANG Qing;WANG Chenghui;YANG Meixiang;SUN Tao;HUANG Ying;TAO Zhu(The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China;Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province;Key Laboratory of Guizhou High Performance Computational Chemistry;College of Tobacco Science,Guizhou University,Guiyang 550025,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2018年第12期2665-2672,共8页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21562015)
贵州省优秀青年科技人才培养计划(批准号:黔教合平台人才[2017]5636)
贵州省普通高等学校科技拔尖人才支持计划项目(批准号:黔教合KY字[2016]059)
贵州省科技厅自然科学基金重点项目(批准号:黔教合JZ字[2014]2005)
贵州省科技厅联合基金项目(批准号:黔教合LH字[2014]7658)
教育部春晖计划合作科研项目(批准号:Z2015002)资助
关键词
八元瓜环
吖啶橙
荧光探针
多菌灵
细胞成像
Cucurbit[8]uril
Acridine orange
Fluorescent probe
Carbendazim
Cell imaging
