大鼠腮腺主导管结扎损伤后再生的初步研究

The regeneration of parotid gland after reopening the ligated main duct in rats

目的:研究由于导管阻塞引起的腮腺组织损伤后的再生过程,并初步探讨其再生机制。方法:54只Wistar大鼠腮腺主导管结扎7 d后再通,再通0、3、5、7、10、14、21和28 d后各取6只鼠,通过HE染色、AB-PAS染色、Western blot检测AQP5和PDCD5蛋白的表达等方法研究大鼠腮腺组织的转归。结果:结扎7 d再通后腮腺组织由萎缩开始增生,腺泡细胞逐渐增多,腺泡细胞与AQP5的表达呈正比,至第14天时基本恢复至正常水平,扩张的导管逐渐减少,导管形态恢复正常。PDCD5的表达呈现先增多后减少的趋势。结论:腮腺组织具有损伤后再生的能力,结扎7 d的腮腺组织再通后可恢复其形态与功能。

Objective:To investigate the regenerative process of injured parotid gland induced by duct ligation.Methods:The main duct of 54 wistar rats was ligated for 7 days and then the lagation was removed.HE staining,AB-PAS staining,Western blot for AQP5 and PDCD5 expression were conducted 0、1、3、5、7、10、14、21、28 days after reopening of the duct(n=6).Results:After reopen,the parotid gland was started regeneration from atrophy.The acinar cells increased.The expression of AQP5 was directly proportional to the acinar cells.The gland tissue recover to the normal level until the 7th day after release of the ligation.The expanded duct reduced,and the duct recover to the normal level 21 days after reopening.The expression of PDCD5 showed a trend of increasing firstly and then decreasing.Conclusion:The parotid gland have the ability of regeneration from injury.The morphology and function of the parotid gland can restored after reopening of the main duct which has been ligated for 7 days.