合肥地区2558例体检女性HPV感染及亚型分布情况

The distribution of HPV infection and subtype distribution in 2 558 females in Hefei area

目的分析合肥地区常规体检女性的人乳头瘤病毒(HPV)感染及亚型分布情况。方法在Luminex 200平台上,用透景27型HPV分型试剂盒对2 558例常规体检女性的宫颈脱落细胞进行HPV基因型检测,按照年龄段分类统计数据,计算感染的阳性率,同时统计其亚型分布特点。结果HPV阳性者397例,阳性率15. 52%。样本被分为4个年龄段组:21～30岁组、31～40岁组、41～50岁组、> 50岁组,阳性率分别为13. 85%、14. 01%、16. 39%和17. 16%;高危型HPV例数与低危型HPV例数的阳性率分别为13. 02%和6. 61%。χ2检验的结果显示,各年龄段之间,总阳性率(χ2=3. 624,P=0. 305)、高危型例数的阳性率(χ2=1. 975,P=0. 578)和低危型例数的阳性率(χ2=4. 507,P=0. 212)差异无统计学意义。各年龄段单一感染率均高于多重感染率。高危型中HPV检出率较高的型别是52 (9. 76%)、16(9. 16%)、58 (6. 77%)、53 (5. 98%),低危型中HPV检出率较高的型别是61 (6. 77%)、81 (5. 78%)、55(5. 38%)。结论合肥地区女性HPV感染的年龄段和型别分布均有不同于其他地区的基因型分布情况,建议本地区人群接种含52、16、58、53型的九价HPV疫苗。

Objective To analyse of human papillomavirus(HPV)infection and distribution of subtypes in women in routine physical examination in Hefei district.Methods The HPV genotypes of 2 558 cases of cervix uteri exfoliated cells were detected on Luminex 200 platform with Tellgen 27 HPV genotyping assay kit.The results were grouped according to the classification of age.Then the positive rates and distribution of subtypes of infection were calculated.Results 397 cases were HPV positive and the positive rate was 15.52%.The samples were divided into 4 age groups,21~30,31~40,41~50 and>50 years old group.The positive rates were 13.85%,14.01%,16.39%and 17.16%respectively.The tests showed that,for the positive rates in all age groups,there was no significant difference in total cases(χ^2=3.624,P=0.305),in the high-risk cases(χ^2=1.975,P=0.578)or in the low-risk cases(χ^2=4.507,P=0.212).Single infection rates were higher than the multiple infection rates in all age groups.The positive detectable rates of high-risk HPV and low-risk HPV were 13.02%and 6.61%respectively.High-risk HPV positive detectable rates were higher in HPV52(9.76%),HPV16(9.16%),HPV58(6.77%)and HPV53(5.98%).While low-risk HPV positive detectable rates were higher in HPV61(6.77%),HPV81(5.78%),HPV55(5.38%).Conclusion The prevalence and type distribution of HPV in Hefei women are different from other regions.It is suggested that nine-valent HPV vaccine which contains HPV 52,16,58 and 53 types should be vaccinated in this region.