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胚胎植入前遗传学筛查在复发性流产及高龄患者中的应用 被引量:6

Application of preimplantation genetic screening in recurrent spontaneous abortion and elderly patients
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摘要 目的探讨胚胎植入前遗传学筛查(PGS)在高龄和复发性流产(RSA)患者中的应用价值。方法选择91例行PGS治疗的高龄和RSA患者作为研究对象,用单细胞全基因组扩增(WGA)结合二代测序技术(NGS)对胚胎23对染色体进行筛查,选择染色体正常的胚胎进行移植,观察其临床结局。结果行PGS检测的526枚胚胎,染色体异常率为67. 49%(355/526),其中RSA患者染色体异常率为65. 96%(217/329),临床妊娠率(CPR)为56. 00%(28/50);高龄患者染色体异常率为72. 84%(177/243),CPR为57. 14%(16/28)。结论高龄和RSA患者胚胎异常率较高,通过PGS可改善其妊娠结局。 Objective To investigate the value of preimplantation genetic screening(PGS)in elderly women and recurrent spontaneous abortion(RSA)patients.Methods 91 elderly and RSA patients who received PGS in our hospital were selected as the study objects.Single cell whole genome amplification combined with next-generation sequencing technique was used to screen 23 pairs of chromosome by embryo biopsy.The embryos with normal chromosome karyotype were selected for transfer,and the clinical outcomes were followed-up.Results Total 526 biopsy embryos in 91 patients were peformed PGS.The abnormal chromosome karyotype rate was 67.49%.The rate of abnormal karyotype in RSA and elderly patients were 65.96%and 72.84%,repecetively,while the clinical pregnancy rate in RSA and elderly patients were 56.00%and 57.14%,repecetively.Conclusion There are higher occurrence of aneuploidy embroys in RSA and/or elderly patients,whose pregnancy outcome can be improved by preimplantation genetic screening.
作者 韩慧敏 舒明明 揣云海 陈梦楠 郝镁娟 商微 封志纯 Han Huimin;Shu Mingming;Chuai Yunhai;CHEN Mengnan;HAO Meijuan;SHANG Wei;FENG Zhichun(Navy Clinical College of Anhui Medical University,Beijing 100048;In Vitro Fertility Center, Dept of Obstetrics&Gynecology,PLA Navy General Hospital,Beijing 100048)
出处 《安徽医科大学学报》 CAS 北大核心 2018年第12期1951-1955,共5页 Acta Universitatis Medicinalis Anhui
基金 首都临床特色应用研究与成果推广(编号:Z151100004015214)
关键词 胚胎植入前遗传学筛查 复发性流产 高龄 preimplantation genetic screening recurrent spontaneous abortion elderly
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