青蒿琥酯通过诱导HO-1产生对结核分枝杆菌诱导的THP-1细胞TNF-α分泌的影响

Effect of HO-1 enhanced by Artesunate on the secretion of cytokine TNF-α induced by mycobacterium tuberculosis-induced THP-1 cells

目的探讨青蒿琥酯(ASN)诱导HO-1产生对结核分枝杆菌(Mtb)诱导的THP-1细胞分泌细胞因子肿瘤坏死因子α(TNF-α)的影响并探讨其可能的作用机制。方法体外培养THP-1细胞,将细胞分为对照组、ASN组、Mtb组、ASN预处理组及Zn PP预处理组。ASN预处理组为浓度0～20μg/ml ASN预处理THP-1细胞4 h,随后加入Mtb(感染复数MOI=10)刺激24 h。锌原卟啉(Zn PP)预处理组为10μmol/L Zn PP抑制剂与THP-1预处理4 h,随后加入Mtb(MOI=10)刺激24 h。MTT法检测ASN对细胞的活性影响。RT-q PCR检测细胞因子TNF-α、核因子E2相关因子2(Nrf2)及血红素氧合酶1(HO-1) mRNA表达; ELISA检测TNF-α蛋白分泌。结果 MTT法检测ASN浓度在0～20μg/ml范围内对细胞无毒性;与对照组相比,Mtb显著增加TNF-αmRNA及蛋白表达,而5～20μg/ml ASN组呈浓度依赖性抑制TNF-αmRNA及蛋白表达(P <0. 05)。此外,ASN能上调Nrf2及HO-1mRNA表达,HO-1竞争性抑制剂Zn PP能增加TNF-α蛋白分泌。结论 ASN通过诱导HO-1产生抑制Mtb诱导的炎症反应。

Objective To investigate the effect of artesunate(ASN)on the secretion of cytokine TNF-αin THP-1 cells induced by Mtb and to investigate its possible mechanism.Methods THP-1 cells were cultured in vitro.The cells were divided into five groups.THP-1 cells were pretreated with 0~20μg/ml ASN for 4h and then stimulated with Mtb(MOI=10)for 24h as ASN pretreatment group.THP-1 cells were pretreated with 10μmol/L ZnPP inhibitor for 4h and then stimulated with Mtb(MOI=10)for 24h as ZnPP pretreatment group.MTT assay was used to detect the effect of ASN on the activity of cells.RT-qPCR was used to detect the mRNA expression of TNF-α,NF-E2-related factor 2(Nrf2)and Heme oxygenase-1(HO-1).ELISA was used to detect the secretion of TNF-αprotein.Results There was no cytotoxicity to cells dealt with the concentrations of 0~20μg/ml of ASN detected by MTT.Compared with the control group,Mtb significantly increased mRNA and protein expression of TNF-α.ASN could inhibit the secretion of TNF-αat mRNA and protein level in a dose-dependent manner(P<0.05).In addition,ASN could up-regulate the mRNA expression of Nrf2 and HO-1.Conclusion ASN can inhibit the inflammatory responses induced by Mtb through up-regulation of HO-1 expression.