广州市售海产品中副溶血弧菌分离菌株的鉴定及其耐药性分析

Isolation,identification and antibiotic resistance analysis of Vibrio parahaemolyticus in seafood from Guangzhou

对8株分离自广州市售海产品中的副溶血弧菌菌株进行鉴定,并分析了其耐药性。于2017年4～5月采集市售海产品,按照国标法对其中的副溶血弧菌进行分离鉴定,并进行16S r DNA同源性比较。采用K-B纸片法和结晶紫染色法分别评估了副溶血弧菌菌株对20种常见抗生素的药物敏感性及其生物膜形成能力。结果表明,8株分离菌株对多粘菌素B、头孢他啶、庆大霉素、强力霉素、氯霉素、红霉素、环丙沙星、萘啶酸这8种抗生素均为敏感,对青霉素、万古霉素2种抗生素均显示耐药,对其他10种抗生素有不同程度的耐药性。相比ATCC17802菌株,分离菌株多重耐药性更为显著,耐抗生素数量为3～10种。分析菌株的生物膜形成能力,发现其耐药性与生物膜形成能力呈正相关。海产品中副溶血弧菌耐药性普遍且多重耐药性显著的现象,应该予以广泛关注和重视。

In this study,eight strains of Vibrio parahaemolyticus isolated from seafoods in Guangzhou were identified and their antibiotic resistances were analyzed.Seafoods were collected from markets in Guangzhou in April to May 2017.According to the detection method of Vibrio parahaemolyticus-GB 4789.7-2013,the physiological and biochemical characteristics of the strains were analyzed,and the strains were also identified by 16S rDNA sequence analysis.The sensitivities of the isolated strains and ATCC 17802 standard strain to 20 antibiotics were evaluated using Kirby-Bauer method.And the biofilm formation of the strain was assessed using crystal violet assay.All isolated strains were found to be susceptible to polymyxin B,ceftazidime,gentamicin,doxycycline,chloramphenicol,erythromycin,ciprofloxacin,and nalidixic acid.Furthermore,they were resistant to penicillin and vancomycin,and showed different degrees of resistance to other antibiotics.Moreover,all strains exhibited more significant multi-drug resistance with range 3 to 10 species antibiotics compared with ATCC 17802.Biofilm formation ability was positively correlated with drug resistance of the strain.The drug resistance of V.parahaemolyticus in seafoods and the significant multi-drug resistance deserve our due attention.