Box-Behnken响应面法优化裸花紫珠有效成分提取工艺

Optimized Extraction of Functional Ingredients fromCallicarpa nudiflora Hook.et Arn.by Box-Behnken Design

为研究裸花紫珠浸膏得率、总黄酮、总酚酸及总皂苷的最佳提取工艺,以浸膏得率和有效成分总含量(总黄酮、总酚酸及总皂苷含量之和)的加权评分值(加权评分值=浸膏得率×0.7+总有效成分含量×0.3)为指标,通过单因素和响应面设计相结合的方法,以提取方法、提取时间、料液比、乙醇浓度、提取次数为考察因素,优化裸花紫珠有效成分提取工艺。结果表明裸花紫珠有效成分的最佳提取工艺为:超声提取时间为42min,料液比1∶17,乙醇体积分数为62%,提取2次。此条件下加权评分值为119.27,预测加权评分值为120.47,两者相差较小。优化后的裸花紫珠有效成分提取工艺简单可行,重现性和可预测性较好。

To optimize the extraction of functional ingredients from the leaves of Callicarpa nudiflora Hook.et Arn.,an experiment based on the Box-Behnken design was conducted.The extraction rate and contents of functional ingredients including flavonoids,phenolic acids,and saponins in the resulting extracts were weighed against the method,duration,cycle,material to solvent ratio,and ethanol concentration of the process.The weighted value for evaluation was calculated by the formula of(dry weight extract 0.7+combined weight of functional ingredients 0.3).The optimized conditions included the application of ultrasound for 42 min,a material:solvent ratio of 1∶17,the use of 62%ethanol for solvent,and two extraction cycles to arrive at a weighted value of 119.27,which was close to the predicted value of 120.47.The established extraction process was simple and applicable with desirable reproducibility and predictability.