沉默长链非编码RNA浆细胞瘤可变易位1基因提高胃癌细胞对阿霉素的化疗敏感性

Knockdown of Long Noncoding RNA Plasmacytoma Variant Translocation 1 Enhances Chemosensitivity of Gastric Cancer Cells to Adriamycin

目的研究长链非编码RNA浆细胞瘤可变易位1 (PVT1)基因调控胃癌细胞对阿霉素化疗敏感性的作用。方法 RNA干扰靶向沉默SGC7901/ADR细胞中PVT1的表达,实时定量PCR检测沉默效果。增强CCK8法检测SGC7901/ADR细胞的增殖活力和化疗药物敏感性;碘化丙啶单染流式细胞术检测SGC7901/ADR细胞的细胞周期;Annexin V-FITC/PI双染流式细胞术检测SGC7901/ADR细胞的凋亡。结果 PVT1沉默能够显著降低阿霉素在SGC7901/ADR细胞中的半数抑制浓度。在0.5μg/mL阿霉素的作用下,PVT1沉默能够抑制SGC7901/ADR细胞的增殖活力,阻滞细胞于G1期,并促进细胞凋亡。PVT1沉默能够提高SGC7901/ADR细胞对阿霉素的化疗敏感性,并提高阿霉素诱导的细胞毒性作用。结论长链非编码RNA PVT1基因与胃癌的化疗耐药相关,PVT1沉默能够提高胃癌耐药细胞对阿霉素的化疗敏感性。

Objective To study the effects of the long noncoding RNA plasmacytoma variant translocation 1(PVT1)on the regulation of chemosensitivity of gastric cancer cells to adriamycin.Methods RNA interference was used to silence the expression of PVT1 in SGC7901/ADR cells.Real-time quantitative PCR was used to detect the effect of silencing.An enhanced CCK8 assay was used to detect the proliferation of PVT1-silenced SGC7901/ADR cells and their sensitivity to the chemotherapeutic drug adriamycin.Cell cycle analysis was performed using propidium iodide(PI)single dye flow cytometry.Apoptosis was detected using Annexin V-fluorescein isothiocyanate/PI double dye flow cytometry.Results Silencing of PVT1 significantly reduced the half-inhibitory concentration of adriamycin in SGC7901/ADR cells.At an adriamycin concentration of 0.5μg/mL,PVT1 silencing inhibited SGC7901/ADR cell proliferation,blocked cells in the G1 phase,and promoted cell apoptosis.PVT1 silencing also enhanced the sensitivity of SGC7901/ADR cells to adriamycin and adriamycin-induced cytotoxicity.Conclusion Long noncoding RNA PVT1 is associated with chemoresistance in gastric cancer.PVT1 silencing can increase the chemosensitivity of drug-resistant gastric cancer cells to adriamycin.