强制性运动疗法结合抗抑郁治疗对脑梗死大鼠神经功能的影响

Effects of Constraint-Induced Movement Therapy Combined with Antidepressant Treatment on Recovery after Cerebral Infarction in Rats

目的探讨强制性运动疗法(CIMT)结合抗抑郁治疗对脑梗死大鼠神经发生及运动功能的影响。方法成年大鼠随机分为4组:假手术组(sham组)、梗死组、梗死后CIMT组(CIMT组)、梗死后CIMT结合抗抑郁治疗组(CIMT+FLUO组)。使用内皮素制作脑梗死大鼠模型。脑梗死后1周使用石膏绷带固定健侧上肢制作CIMT模型,CIMT组CIMT进行3周。CIMT+FLUO组脑梗死后1周,大鼠氟西汀(16 mg/kg)口服,持续3周。使用免疫荧光染色检测DCX和BrdU双染阳性细胞表达,应用梯形平衡木测试大鼠的肢体运动功能。结果与Sham组比较,脑梗死组大鼠侧脑室下区新生神经细胞明显增多(P <0.01)。与脑梗死组比较,CIMT组侧脑室下区新生神经细胞进一步增多(P <0.01);同时运动功能恢复(P <0.05)。而CIMT+FLUO组与CIMT组比较,新生神经细胞及运动功能恢复没有统计学差异(P> 0.05)。结论 CIMT能够促进大鼠脑梗死后神经发生及运动功能恢复,联合使用抗抑郁治疗未出现明确的促进作用。

Objective To determine whether constraint-induced movement therapy(CIMT)combined with antidepressant treatment could influence neurogenesis and recovery after cerebral infarction in rats.Methods Adult rats were divided into a sham group,an infarction group,an infarction treated with CIMT group(CIMT group),and an infarction treated with CIMT+fluoxetine(FLUO)group(CIMT+FLUO group).Infarction was induced with endothelin-1(ET-1).The healthy forelimbs of rats in the CIMT group were restrained in plaster casts.Rats in the CIMT+FLUO group consumed water with fluoxetine for 3 weeks.The expression of doublecortin and 3,3’-diaminobenzidine was measured using immunohistochemistry techniques,and motor function was evaluated with a beam walking test.Results CIMT enhanced the proliferation of neuroblasts(P<0.01)and behavioral recovery(P<0.05).CIMT combined with antidepressant treatment in CIMT+FLUO group had no effect on neurogenesis and recovery compared with CIMT group(all P>0.05).Conclusion CIMT could enhance the neurogenesis and recovery after cerebral infarction,and there are nofurther promotion on CIMT combined with antidepressant treatment.